临床血清替考拉宁检测的质量：一项欧洲实验性EQA分布情况

The quality of clinical serum teicoplanin assays: an experimental European EQA distribution.

作者信息

White L O, McMullin C, Davis A J, MacGowan A P, Harding I, Reeves D S

机构信息

UK NEQAS for Antibiotic Assays, Southmead Hospital Bristol, UK.

出版信息

J Antimicrob Chemother. 1996 Oct;38(4):701-6. doi: 10.1093/jac/38.4.701.

DOI:10.1093/jac/38.4.701

PMID:8937964

Abstract

Two sets of six samples of human serum spiked with predetermined concentrations of teicoplanin were circulated, 6 months apart, to 22 European laboratories for the purposes of external quality assessment (EQA). The laboratories returned assay results using either bioassay, high-performance liquid chromatography (HPLC) or fluorescence polarization immunoassay (FPIA). FPIA was the most popular method and performance was generally satisfactory. Some laboratories using HPLC or microbiological assay performed satisfactorily but others did not. Only seven laboratories (32%) showed consistently satisfactory performance. There appears to be a need for continuing EQA of clinical teicoplanin assays.

摘要

两组分别含有预先设定浓度替考拉宁的六份人血清样本，相隔6个月分发给22家欧洲实验室，用于外部质量评估（EQA）。这些实验室使用生物测定法、高效液相色谱法（HPLC）或荧光偏振免疫测定法（FPIA）返回检测结果。FPIA是最常用的方法，其性能总体令人满意。一些使用HPLC或微生物测定法的实验室表现令人满意，但其他实验室则不然。只有七家实验室（32%）始终表现令人满意。看来有必要对临床替考拉宁检测进行持续的EQA。