Furutani M, Arii S, Higashitsuji H, Mise M, Niwano M, Harada T, Nakayama H, Fukumoto M, Imamura M, Fujita J
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Hepatology. 1996 Dec;24(6):1441-5. doi: 10.1053/jhep.1996.v24.pm0008938178.
We identified kan-1 complementary DNA (cDNA), the sequence of which is identical to bile acid CoA:amino acid N-acyltransferase (BAT), a liver enzyme that catalyzes the conjugation of bile acids with glycine or taurine. Kan-1(BAT) messenger RNA (mRNA) levels of the resected specimens obtained from 37 hepatocellular carcinoma (HCC) patients were studied in an attempt to evaluate prognostic significance in HCC patients after partial hepatectomy. Using Northern blot hybridization, kan-1(BAT) mRNA levels were quantified in tumorous and nontumorous tissues, and the ratio of the former to the latter was defined as the kan-1(BAT) ratio. Twelve patients had a kan-1(BAT) ratio < 0.5 (low kan-1[BAT] ratio), and 25 patients had a ratio >0.5 (high kan-1[BAT] ratio). The patients with a low kan-1(BAT) ratio demonstrated poorer survival than the patients with a high kan-1(BAT) ratio (P = .0013). The overall estimated hazard ratio for death in patients with a low kan-1(BAT) ratio was 68.05 according to a multivariate model (P = .0005). Thus, the kan-1(BAT) ratio may serve as a new molecular prognostic marker in HCC patients, following hepatic resection.
我们鉴定出了kan-1互补DNA（cDNA），其序列与胆汁酸辅酶A：氨基酸N-酰基转移酶（BAT）相同，BAT是一种肝脏酶，可催化胆汁酸与甘氨酸或牛磺酸的结合。我们研究了37例肝细胞癌（HCC）患者切除标本的Kan-1（BAT）信使核糖核酸（mRNA）水平，以评估部分肝切除术后HCC患者的预后意义。使用Northern印迹杂交法，对肿瘤组织和非肿瘤组织中的Kan-1（BAT）mRNA水平进行定量，并将前者与后者的比值定义为Kan-1（BAT）比值。12例患者的Kan-1（BAT）比值<0.5（低Kan-1[BAT]比值），25例患者的比值>0.5（高Kan-1[BAT]比值）。Kan-1（BAT）比值低的患者生存率低于Kan-1（BAT）比值高的患者（P = 0.
