Nilsson T, Cantera L, Edvinsson L
Division of Experimental Vascular Research, Department of Internal Medicine, Lund University Hospital, Sweden.
Neurosci Lett. 1996 Feb 9;204(3):145-8. doi: 10.1016/0304-3940(96)12315-6.
The neuropeptide Y-induced vasoconstriction of human cerebral arteries is mediated by the neuropeptide Y Y1 receptor. We conclude this on the basis of our results from: (1) in vitro studies on neuropeptide Y agonists. Neuropeptide Y and pro34NPY caused potent and long-lasting contractions of human cerebral arteries, while NPY 13-36 had no contractile effect at all on the vessels tested; (2) in vitro studies using the selective Y1 receptor antagonist BIBP3226 which in increasing concentrations (10(-9)-10(-6)M) caused a parallel shift to the right of the neuropeptide Y concentration-response curve without change of the maximum contractile response (pA2 value 8.38 +/- 0.10); and (3) with reverse transcriptase-polymerase chain reaction (RT-PCR) we detected specific mRNA for a neuropeptide Y Y1 receptor in human pial and human middle cerebral arteries using three forward primers and one reverse primer.
神经肽Y诱导的人脑动脉血管收缩是由神经肽Y Y1受体介导的。我们基于以下结果得出这一结论：（1）对神经肽Y激动剂的体外研究。神经肽Y和Pro34NPY可引起人脑动脉强烈且持久的收缩，而NPY 13 - 36对所测试的血管完全没有收缩作用；（2）使用选择性Y1受体拮抗剂BIBP3226的体外研究，该拮抗剂浓度增加时（10^(-9)-10^(-6)M），神经肽Y浓度 - 反应曲线向右平行移动，最大收缩反应无变化（pA2值为8.38±0.10）；（3）通过逆转录 - 聚合酶链反应（RT - PCR），我们使用三个正向引物和一个反向引物在人软脑膜和大脑中动脉中检测到神经肽Y Y1受体的特异性mRNA。
