Van Vyncht G, Preece S, Gaspar P, Maghuin-Rogister G, DePauw E
Chemistry Department, University of Liège, Sart Tilman, Belgium.
J Chromatogr A. 1996 Oct 25;750(1-2):43-9. doi: 10.1016/0021-9673(96)00540-7.
beta-Agonists are substances used in veterinary and human medicine for the treatment of pulmonary disorders. They have found a use as growth promoters to improve meat-to-fat ratios in cattle but they are not authorized for use in the European Union. Due to their presence in trace levels (usually less than 1 microgram/kg), to the diversity of the illegally used compounds and to the complexity of the biological matrices analysed, the detection of these residues requires a very sensitive and specific method of determination. This work describes the strategy of analysis we developed for five beta-agonists in urine and liver. The combination of improved solid- or liquid-phase extraction methods and LC or GC-MS-MS (in the multiple reaction monitoring mode) has shown to provide a system suitable for the control of these substances. The efficiency of extraction and the sensitivity and selectivity allow this multiresidue detection down to, and below, the UK regulatory level of 0.5 microgram/kg. Moreover, the use of LC removes the need for the derivatisation step (cyclic methylboronate derivatives) which is required prior to GC-MS-MS analysis.
β-激动剂是兽医学和人类医学中用于治疗肺部疾病的物质。它们已被用作生长促进剂以改善牛的肉脂比，但在欧盟未获批准使用。由于它们以痕量水平存在（通常低于1微克/千克），非法使用的化合物种类繁多，且所分析的生物基质复杂，因此检测这些残留物需要一种非常灵敏且特异的测定方法。这项工作描述了我们针对尿液和肝脏中的五种β-激动剂开发的分析策略。改进的固相或液相萃取方法与液相色谱或气相色谱-串联质谱（多反应监测模式）相结合，已证明可提供一个适用于这些物质控制的系统。萃取效率以及灵敏度和选择性使得这种多残留检测能够达到并低于英国0.5微克/千克的监管水平。此外，液相色谱的使用无需气相色谱-串联质谱分析之前所需的衍生化步骤（环状甲基硼酸酯衍生物）。
