Saito T R, Hokao R, Wakafuji Y, Igarashi N, Agematsu Y, Takahashi K W
Department of Laboratory Animal Science, Nippon Veterinary and Animal Science University, Tokyo, Japan.
Lab Anim. 1996 Oct;30(4):332-6. doi: 10.1258/002367796780739844.
The present study was undertaken to determine sperm motility and counts in semen yielded by para-chloroamphetamine (PCA)-induced ejaculation of aged rats which had lost their reproductive ability, and to attempt artificial insemination with suspensions of spermatozoa obtained in this way. The semen yielded by PCA-induced ejaculation from aged (75-week-old) rats had average sperm counts of 0.82 +/- 0.69 x 10(7), which were much lower than the average counts (9.42 +/- 1.65 x 10(7)) for semen spontaneously ejaculated by young adult rats (14 weeks old). However, 77.5% of the spermatozoa contained in the PCA-induced semen were rated as showing the most active movement. Spermatozoa collected in this way were injected into the upper parts of both uterine horns or into both ovarian bursae. Both methods made the females pregnant, but the results were better after injection into the ovarian bursae. The offspring born to these females showed no abnormalities.
本研究旨在测定对已丧失生殖能力的老年大鼠进行对氯苯丙胺（PCA）诱导射精所获精液中的精子活力和数量，并尝试用以此法获得的精子悬浮液进行人工授精。对老年（75周龄）大鼠进行PCA诱导射精所获精液的平均精子数量为0.82±0.69×10⁷，远低于年轻成年大鼠（14周龄）自然射精精液的平均数量（9.42±1.65×10⁷）。然而，PCA诱导精液中的精子有77.5%被评为具有最活跃的运动。以此法收集的精子被注入双侧子宫角上部或双侧卵巢囊。两种方法均使雌性大鼠受孕，但注入卵巢囊后的效果更好。这些雌性大鼠所生后代未显示出异常。
