Tocher D R, Bell J G, Sargent J R
NERC Unit of Aquatic Biochemistry, Department of Biological and Molecular Sciences, University of Stirling, Scotland, UK.
Comp Biochem Physiol B Biochem Mol Biol. 1996 Oct;115(2):215-22. doi: 10.1016/0305-0491(96)00113-7.
Primary cultures of turbot (Scophthalmus maximus) brain astroglial cells established in medium containing fetal bovine serum contain increased proportions of 18:1(n-9), total (n-9) and (n-6) polyunsaturated fatty acids (PUFA) and greatly reduced (n-3) PUFA in comparison with turbot brain. Supplementation with a mixture of 5 microM eicosapentaenoic [20:5(n-3)] and 25 microM docosahexaenoic [22:6(n-3)] acids for 4 days significantly increased the percentages of these acids in total cellular lipid of turbot astrocytes and restored the (n-3) PUFA composition of the cells to that found in turbot brain. The production of prostaglandins (PG) E and F of the 2- and 3-series and leukotrienes (LT) C4 and C5 in response to various agonists was determined in PUFA-supplemented astrocytes. Calcium ionophore A23187, platelet activating factor and substance P stimulated the production of both PGF and PGE. Interleukin-1 beta significantly stimulated the production of PGF only. There were differences between the agonists in their effects on the relative levels of 2- and 3-series PGs produced. Only very low amounts of LTC were produced by the turbot astrocytes, with only substance P showing a minor stimulatory effect.
与大菱鲆脑相比，在含有胎牛血清的培养基中建立的大菱鲆（Scophthalmus maximus）脑星形胶质细胞原代培养物中，18:1(n-9)、总(n-9)和(n-6)多不饱和脂肪酸（PUFA）的比例增加，而(n-3) PUFA大幅减少。用5 microM二十碳五烯酸[20:5(n-3)]和25 microM二十二碳六烯酸[22:6(n-3)]的混合物补充4天，显著增加了这些酸在大菱鲆星形胶质细胞总细胞脂质中的百分比，并使细胞的(n-3) PUFA组成恢复到在大菱鲆脑中发现的水平。在补充了PUFA的星形胶质细胞中，测定了2-和3-系列前列腺素（PG）E和F以及白三烯（LT）C4和C5对各种激动剂的反应。钙离子载体A23187、血小板活化因子和P物质刺激了PGF和PGE的产生。白细胞介素-1β仅显著刺激了PGF的产生。激动剂对产生的2-和3-系列PG的相对水平的影响存在差异。大菱鲆星形胶质细胞仅产生极少量的LTC，只有P物质显示出轻微的刺激作用。
