Barral V, Morand S, Pointier J P, Théron A
Laboratoire de Biologie Animale, UMR 5555 du CNRS, Université 52, Perpignan, France.
Parasitology. 1996 Dec;113 ( Pt 6):511-7. doi: 10.1017/s003118200006755x.
Random amplified polymorphic DNA markers (RAPD) were used to visualize the genetic diversity within and between infrapopulations of Schistosoma mansoni recovered from the natural vertebrate host, Rattus rattus, trapped at an insular Guadeloupean focus. Phenotypes were characterized by the sex of the parasites and by 8 polymorphic markers generated by 3 primers. Among the 212 parasite individuals recovered from 10 infected rats, 78 genotypes were characterized. All the hosts naturally infected harboured multiple parasite genotypes with a maximum diversity of 28 genotypes/host. Phenotypic and genotypic diversity calculated by Shannon-Wiener's indices and Lynch and Milligan's estimators respectively is, on average, greater within than between hosts. Considering the very low snail infection rates observed in this focus and the rapid turnover of the vertebrate hosts, our results suggest that the high mobility of the vertebrate host and/or plurimiracidial snail infections could be factors responsible for parasite genetic diversity within hosts.
随机扩增多态性DNA标记（RAPD）被用于观察从自然脊椎动物宿主——在瓜德罗普岛一个岛屿疫源地捕获的黑家鼠体内回收的曼氏血吸虫群体内和群体间的遗传多样性。表型通过寄生虫的性别以及由3种引物产生的8个多态性标记来表征。从10只受感染大鼠体内回收的212个寄生虫个体中，鉴定出了78种基因型。所有自然感染的宿主都携带多种寄生虫基因型，每个宿主的最大多样性为28种基因型。分别通过香农 - 维纳指数以及林奇和米利根估计器计算的表型和基因型多样性，平均而言，宿主内的多样性大于宿主间的多样性。考虑到在该疫源地观察到的极低的钉螺感染率以及脊椎动物宿主的快速更替，我们的结果表明脊椎动物宿主的高流动性和/或多尾蚴钉螺感染可能是宿主内寄生虫遗传多样性的原因。
