Calhoun W J, Jarjour N N, Gleich G J, Schwartz L B, Busse W W
Division of Pulmonary, Allergy, and Critical Care Medicine, University of Pittsburgh, PA 15261, USA.
J Allergy Clin Immunol. 1996 Nov;98(5 Pt 2):S46-50; discussion S64-6.
In many patients with asthma, exposure to allergen results in worsening symptoms, pulmonary dysfunction, and increased airway inflammation. In this study, segmental allergen challenge and bronchoalveolar lavage were used to evaluate the airway responses at 5 minutes and 48 hours and to study the extent of inhibition of the response by pretreatment with nedocromil sodium in eight subjects with allergic asthma in a double-blind, placebo-controlled trial. At 5 minutes after challenge, nedocromil sodium pretreatment significantly reduced histamine concentrations, with a trend toward a concomitant reduction in tryptase levels. Nedocromil sodium did not affect the increase in total protein, total cell counts, or cell concentrations that occurred 48 hours after challenge, but it did significantly reduce eosinophil recruitment. Eosinophil activation, assessed as release of granule proteins, was unaffected. A significant positive correlation was shown between the degree of histamine reduction at 5 minutes and the degree of reduction of eosinophil influx at 48 hours, raising the possibility that eosinophil influx into the airway may depend on mediators or cytokines released during the immediate response to allergen.
在许多哮喘患者中，接触变应原会导致症状加重、肺功能障碍及气道炎症增加。在本研究中，采用双盲、安慰剂对照试验，对8名过敏性哮喘患者进行节段性变应原激发试验和支气管肺泡灌洗，以评估5分钟和48小时时的气道反应，并研究奈多罗米钠预处理对反应的抑制程度。激发试验后5分钟，奈多罗米钠预处理显著降低组胺浓度，同时有使类胰蛋白酶水平降低的趋势。奈多罗米钠不影响激发试验后48小时出现的总蛋白、总细胞计数或细胞浓度的增加，但它确实显著减少了嗜酸性粒细胞募集。以颗粒蛋白释放来评估的嗜酸性粒细胞活化未受影响。5分钟时组胺降低程度与48小时时嗜酸性粒细胞流入减少程度之间呈显著正相关，这增加了嗜酸性粒细胞流入气道可能取决于变应原即刻反应期间释放的介质或细胞因子的可能性。
