Gladson C L
Department of Pathology, University of Alabama at Birmingham 35294, USA.
J Neuropathol Exp Neurol. 1996 Nov;55(11):1143-9. doi: 10.1097/00005072-199611000-00005.
Angiogenesis, which promotes tumor proliferation and invasiveness, is mediated by integrin-dependent cell adhesion mechanisms and requires expression of integrin alpha v beta 3. To determine whether integrin alpha v beta 3 is expressed in the small blood vessel hyperplasia characteristic of glioblastoma tumors, we explored the mRNA and protein expression of integrin alpha v, beta 3, beta 5, and beta 1 subunits in small blood vessels in gliomas of various grades and nontumorous brain biopsies. Antisense beta 3 and beta 5 riboprobes hybridized to small blood vessels (endothelial and adjacent mesenchymal cells) in 11 of 11 glioblastomas and 2 of 4 anaplastic astrocytomas, but failed to hybridize to small blood vessels in 12 non-neoplastic specimens and 4 low-grade astrocytomas. In contrast, antisense alpha v and beta 1 riboprobes hybridized to small blood vessels in all of the biopsies. The expression of integrin alpha v beta 3 protein in these vessels was determined by immunohistochemical analysis using anti-integrin subunit-specific antibodies. The findings were similar to those obtained using in situ hybridization: beta 3 subunit protein was detected on small blood vessels in 9 of 12 glioblastoma and 2 of 4 anaplastic astrocytomas, whereas it was not detected in small blood vessels of 10 non-neoplastic brains and 4 low-grade astrocytomas. In contrast, the alpha v and beta 1 subunit proteins were detected in small blood vessels in all of the brain biopsies, whereas the beta 5 subunit protein could not be detected. These data suggest that integrin alpha v beta 3 is expressed in small blood vessels of glioblastoma tumors.
促进肿瘤增殖和侵袭的血管生成由整合素依赖性细胞黏附机制介导，并且需要整合素αvβ3的表达。为了确定整合素αvβ3是否在胶质母细胞瘤特征性的小血管增生中表达，我们探究了不同级别胶质瘤和非肿瘤性脑活检组织中小血管中整合素αv、β3、β5和β1亚基的mRNA和蛋白表达。反义β3和β5核糖探针与11例胶质母细胞瘤中的11例以及4例间变性星形细胞瘤中的2例的小血管（内皮细胞和相邻间充质细胞）杂交，但未与12例非肿瘤标本和4例低级别星形细胞瘤中的小血管杂交。相比之下，反义αv和β1核糖探针与所有活检组织中的小血管杂交。使用抗整合素亚基特异性抗体通过免疫组织化学分析确定这些血管中整合素αvβ3蛋白的表达。结果与原位杂交相似：在12例胶质母细胞瘤中的9例以及4例间变性星形细胞瘤中的2例的小血管中检测到β3亚基蛋白，而在10例非肿瘤性脑和4例低级别星形细胞瘤的小血管中未检测到。相比之下，在所有脑活检组织的小血管中均检测到αv和β1亚基蛋白，而未检测到β5亚基蛋白。这些数据表明整合素αvβ3在胶质母细胞瘤的小血管中表达。
