Palczynski C, Krakowiak A, Ruta U, Krajewska B, Rudzinski J, Gielec L, Górski P
Department of Occupational Diseases, Nofer Institute of Occupational Medicine, Lódz, Poland.
Allergol Immunopathol (Madr). 1996 Jul-Aug;24(4):163-8.
Ten patients with bronchial asthma and allergy to house-dust mite (HDM) and ten normal, nonatopic control subjects underwent a bronchial challenge with flour. Before and 24 h after the allergen provocation with flour, the levels of eosinophil cationic protein (ECP) and myeloperoxidase (MPO) were determined in the serum and nasal lavage fluid. All allergics showed an isolated immediate asthmatic reaction (IAR). After the flour challenge only in asthmatic patients the increase was detected in the mean values of: 1/eosinophils (mean value before 16.7 x 10(3)/mm3; mean after; 10 min 132.9 x 10(3)/ml; 3 hr 183.6 x 10(3)/mm3; 24 h 110.6 x 103/mm3, p < 0.05), 2/basophils (mean before 1.2 x 10(3)/mm3; mean after: 10 min 5.3 x 10(3)/ml; 3 h s 14.1 x 10(3)/mm3 24 h was 18.3/mm3, p < 0.05), 3/neutrophils (mean before 9.2 x 10(3)/mm3; mean after 24 h 18.2 x 10(3)/mm3, p < 0.05) in the nasal lavage fluid. In contrast to a group of normal subjects, asthmatics were found to have higher postchallenge levels of ECP and MPO in the nasal secretions as compared with the prechallenge levels (ECP-mean 3.85 ug/l compared with 32.17 ug/l p < 0.05; MPO-mean 120.02 ug/l compared with 1313.2 ug/l, p < 0.05). The authors did not find any significant difference between pre- and postchallenge levels of ECP and MPO in the serum of asthmatics and controls. The higher levels of MPO as well as higher count of neutrophils observed in asthmatic patients 24 h after allergen challenge support the neutrophil involvement in the allergic inflammation. Our results indicate that both neutrophils and eosinophils take part in allergic reaction in the mucosa.
10名对屋尘螨（HDM）过敏的支气管哮喘患者和10名正常、非特应性对照受试者接受了面粉支气管激发试验。在面粉变应原激发前和激发后24小时，测定血清和鼻腔灌洗液中嗜酸性粒细胞阳离子蛋白（ECP）和髓过氧化物酶（MPO）的水平。所有过敏患者均出现孤立的速发型哮喘反应（IAR）。面粉激发后，仅哮喘患者鼻腔灌洗液中的以下指标平均值升高：1/嗜酸性粒细胞（激发前平均值为16.7×10³/mm³；激发后，10分钟时为132.9×10³/ml；3小时时为183.6×10³/mm³；24小时时为110.6×10³/mm³，p<0.05），2/嗜碱性粒细胞（激发前平均值为1.2×10³/mm³；激发后，10分钟时为5.3×10³/ml；3小时时为14.1×10³/mm³；24小时时为18.3/mm³，p<0.05），3/中性粒细胞（激发前平均值为9.2×10³/mm³；激发后24小时为18.2×10³/mm³，p<0.05）。与正常受试者组相比，哮喘患者激发后鼻腔分泌物中ECP和MPO水平高于激发前水平（ECP-平均值3.85μg/l，激发前为32.17μg/l，p<0.05；MPO-平均值120.02μg/l，激发前为1313.2μg/l，p<0.05）。作者未发现哮喘患者和对照者血清中激发前和激发后ECP和MPO水平有任何显著差异。变应原激发后24小时哮喘患者中观察到的较高MPO水平以及较高的中性粒细胞计数支持中性粒细胞参与了变应性炎症。我们的结果表明，中性粒细胞和嗜酸性粒细胞均参与了黏膜中的变应性反应。
