蛋白质的噬菌体展示

Phage display of proteins.

作者信息

Dunn I S

机构信息

Queensland Cancer Fund Research Unit, Department of Patholog,y Medical School, University of Queensland, Herston 4006, Brisbane, Queensland, Australia.

出版信息

Curr Opin Biotechnol. 1996 Oct;7(5):547-53. doi: 10.1016/s0958-1669(96)80060-7.

DOI:10.1016/s0958-1669(96)80060-7

PMID:8939620

Abstract

Phage display of proteins continues to be an important technology with a variety of applications. In the past year, advances have been made in coupling rational protein design with the power of the display selection process. In addition to the widely used filamentous phage, other bacteriophage surface expression systems have now been developed, some of which may be of particular use for the selection of surface-display cDNA clones.

摘要

蛋白质的噬菌体展示仍然是一项具有多种应用的重要技术。在过去的一年里，将合理的蛋白质设计与展示筛选过程的强大功能相结合方面取得了进展。除了广泛使用的丝状噬菌体，现在还开发了其他噬菌体表面表达系统，其中一些可能对表面展示cDNA克隆的筛选特别有用。