Bartek J, Bartkova J, Lukas J
Danish Cancer Society, Division of Cancer Biology, Strandboulevarden 49, DK-2100 Copenhagen, Denmark.
Curr Opin Cell Biol. 1996 Dec;8(6):805-14. doi: 10.1016/s0955-0674(96)80081-0.
The emerging role of the retinoblastoma protein (pRb) as a major controller of the restriction point has been supported by recent discoveries, including pRb's ability to repress gene transcription by all three RNA polymerases, which suggests a link between DNA replication and cell growth. Convergent genetic and biochemical data provide new insights into the molecular events that are upstream of, at, and downstream of pRb phosphorylation, which is regulated by G1-phase cyclins and cyclin-dependent kinases (Cdks) and their inhibitors (CKIs). Major advances have also been made in our understanding of a key role of the pathway involving cyclin D, Cdks, CKIs, pRb and E2F both in commitment to traversing the cell cycle and in restraining oncogenesis.
视网膜母细胞瘤蛋白（pRb）作为限制点主要调控因子这一新兴作用已得到近期多项发现的支持，包括pRb通过所有三种RNA聚合酶抑制基因转录的能力，这暗示了DNA复制与细胞生长之间的联系。趋同的遗传学和生物化学数据为pRb磷酸化上游、磷酸化过程及下游的分子事件提供了新见解，pRb磷酸化受G1期细胞周期蛋白、细胞周期蛋白依赖性激酶（Cdks）及其抑制剂（CKIs）调控。在我们对涉及细胞周期蛋白D、Cdks、CKIs、pRb和E2F的信号通路在驱动细胞周期进程和抑制肿瘤发生中的关键作用的理解方面也取得了重大进展。
