Suck R, Zeltz P, Falk J, Acker A, Kössel H, Krupinska K
Botanisches Institut der Universität zu Köln, Gyrhofstrasse 15, D-50931 Köln, Germany.
Curr Genet. 1996 Dec;30(6):515-21. doi: 10.1007/s002940050164.
Transcriptionally active chromosomes (TACs) were isolated from mature chloroplasts of barley, from proplastids enriched in basal segments of barley primary foliage leaves, and from ribosome-deficient plastids of heat-bleached barley leaves. Immunological analysis with a specific antibody raised against the plastid rpoA gene product revealed that chloroplasts contain an immunoreactive protein of 38 kDa in the TAC fraction which appears to be identical to the alpha-subunit contained in the soluble RNA polymerase (sRNAP) fraction of the same chloroplasts. However, only traces of immunoreactive protein were detected in a TAC preparation derived from "proplastids". A positive correlation could be demonstrated between transcriptional activity and the amount of immunoreactive 38-kDa protein by analyzing different TAC fractions eluting at different times during gel filtration of a standard TAC preparation as well as in TAC preparations obtained under various detergent conditions.
转录活性染色体（TACs）从大麦成熟叶绿体、富含大麦初生叶基部片段的前质体以及热漂白大麦叶的核糖体缺陷型质体中分离得到。用针对质体rpoA基因产物产生的特异性抗体进行免疫分析表明，叶绿体在TAC组分中含有一种38 kDa的免疫反应性蛋白，该蛋白似乎与同一叶绿体的可溶性RNA聚合酶（sRNAP）组分中所含的α亚基相同。然而，在源自“前质体”的TAC制剂中仅检测到痕量的免疫反应性蛋白。通过分析标准TAC制剂凝胶过滤过程中不同时间洗脱的不同TAC组分以及在各种去污剂条件下获得的TAC制剂，可证明转录活性与免疫反应性38 kDa蛋白的量之间存在正相关。
