Manzerra P, Brown I R
Department of Zoology, University of Toronto, Ontario, Canada.
Exp Cell Res. 1996 Nov 25;229(1):35-47. doi: 10.1006/excr.1996.0341.
Two characteristic features of the heat shock response, (i) induction of hsp70 protein and (ii) nuclear translocation of constitutive hsc70 and stress-inducible hsp70 protein, were utilized as markers of cellular stress in the rabbit brain. Following a physiologically relevant increase in body temperature of 2.7 +/- .3 degrees C, nonneuronal cell types, such as ependymal cells and oligodendrocytes, undergo a stress response as assayed by the above criteria. In contrast, several neuronal cell populations required an increased degree of hyperthermic stress (3.4 +/- .2 degrees C) before exhibiting nuclear translocation of constitutive hsc70 protein. Induction of hsp70 protein was not observed in these neuronal cells at either temperature. The present results suggest that certain neurons in the rabbit brain are buffered against induction of the heat shock response, perhaps due to their high constitutive levels of hsc70 protein.
热休克反应的两个特征性表现，即（i）hsp70蛋白的诱导和（ii）组成型hsc70及应激诱导型hsp70蛋白的核转位，被用作兔脑细胞应激的标志物。在体温生理性升高2.7±0.3摄氏度后，通过上述标准检测发现，诸如室管膜细胞和少突胶质细胞等非神经元细胞类型会发生应激反应。相比之下，几个神经元细胞群体在组成型hsc70蛋白出现核转位之前，需要更高程度的热应激（3.4±0.2摄氏度）。在这两个温度下，这些神经元细胞均未观察到hsp70蛋白的诱导。目前的结果表明，兔脑中的某些神经元对热休克反应的诱导具有缓冲作用，这可能是由于它们的hsc70蛋白具有较高的组成型水平。
