Nankova B, Kvetnansky R, Hiremagalur B, Sabban B, Rusnak M, Sabban E L
Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595, USA.
Endocrinology. 1996 Dec;137(12):5597-604. doi: 10.1210/endo.137.12.8940389.
Sympathetic ganglia are the major contributors to the stress-elicited rise in circulating norepinephrine, enkephalins, and neuropeptide Y. Here we examined the effect of immobilization stress and treatment with ACTH and glucocorticoids on messenger RNA (mRNA) levels for tyrosine hydroxylase (TH), dopamine beta-hydroxylase (DBH), preproneuropeptide Y (pre-NPY), and proenkephalin in rat superior cervical ganglia (SCG) and in stellate ganglia. Our results show a severalfold increase in the relative abundance of TH and NPY mRNAs in response to a single immobilization. Repeated stress elevated expression of all the genes studied and increased TH immunoreactivity in both ganglia. The effect of stress was more pronounced in SCG. Prolonged cortisol administration failed to alter the mRNA levels of TH, DBH, and NPY in control animals but attenuated the response to stress. In contrast, TH and DBH mRNA levels in the SCG, but not in adrenal medulla, were elevated by ACTH administration, similar to the levels attained after immobilization. The results revealed that the regulation of gene expression in response to immobilization stress in sympathetic neurons differs from the regulation in adrenal medulla. The study implicates hormonal involvement in the stress-induced changes in TH, DBH, NPY, and proenkephalin gene expression in sympathetic ganglia.
交感神经节是应激引发循环中去甲肾上腺素、脑啡肽和神经肽Y升高的主要贡献者。在此，我们研究了制动应激以及促肾上腺皮质激素（ACTH）和糖皮质激素处理对大鼠颈上神经节（SCG）和星状神经节中酪氨酸羟化酶（TH）、多巴胺β-羟化酶（DBH）、前神经肽Y（pre-NPY）和前脑啡肽信使核糖核酸（mRNA）水平的影响。我们的结果显示，单次制动后，TH和NPY mRNA的相对丰度增加了数倍。反复应激提高了所有研究基因的表达，并增加了两个神经节中TH的免疫反应性。应激在SCG中的作用更为明显。长期给予皮质醇未能改变对照动物中TH、DBH和NPY的mRNA水平，但减弱了对应激的反应。相反，给予ACTH可使SCG中TH和DBH的mRNA水平升高，肾上腺髓质中则不然，这与制动后达到的水平相似。结果表明，交感神经元对应激的基因表达调控与肾上腺髓质中的调控不同。该研究表明，激素参与了交感神经节中应激诱导的TH、DBH、NPY和前脑啡肽基因表达的变化。
