Ida M, Kamada A
Department of Biochemistry, Osaka Dental University, Japan.
J Osaka Dent Univ. 1995 Oct;29(2):39-50.
We investigated changes in the glycosaminoglycans (GAGs) during progression of a human gingival carcinoma xenograft line, GK -1, in nude mice. The GAGs extracted from cancers 3, 5, 7, 10 and 15 weeks after transplantation consisted of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS) as major components, and dermatan sulfate (DS) as a trace component for all cancers. HPLC analysis revealed that the HA content per defatted tissue dry weight increased in the cancers 5 weeks after transplantation compared to those of 3 weeks (p < 0.05), while CS for cancers at 10 weeks decreased compared with 7 weeks (p < 0.05). However, HS showed no significant change. Both the CS and DS contained primarily 4-sulfated disaccharide units. Immunohistochemical staining with antibody 2-B-6 for the PGs having delta DI-4S produced by chondroitinase ABC digestion showed that CS is located in the tissue surrounding the cancer nests and mass. These results indicate that the location of accumulation of CS, which primarily contains 4-sulfated disaccharide units, plays an important role in cancer progression.
我们研究了人牙龈癌异种移植瘤系GK-1在裸鼠体内生长过程中糖胺聚糖（GAGs）的变化。移植后3、5、7、10和15周从肿瘤中提取的GAGs主要成分包括透明质酸（HA）、硫酸软骨素（CS）和硫酸乙酰肝素（HS），所有肿瘤中的硫酸皮肤素（DS）均为微量成分。高效液相色谱分析显示，与移植后3周的肿瘤相比，移植后5周的肿瘤中每脱脂组织干重的HA含量增加（p<0.05），而10周肿瘤中的CS含量与7周相比有所下降（p<0.05）。然而，HS没有显著变化。CS和DS主要都含有4-硫酸化二糖单元。用抗体2-B-6对经软骨素酶ABC消化产生的具有δDI-4S的蛋白聚糖进行免疫组织化学染色，结果显示CS位于癌巢和肿块周围的组织中。这些结果表明，主要含有4-硫酸化二糖单元的CS的积累位置在癌症进展中起重要作用。
