Theocharis A D, Tsara M E, Papageorgacopoulou N, Karavias D D, Theocharis D A
Department of Chemistry, University of Patras, Patras, Greece.
Biochim Biophys Acta. 2000 Oct 18;1502(2):201-6. doi: 10.1016/s0925-4439(00)00051-x.
The amount and the types of glycosaminoglycans (GAGs) present in human pancreatic carcinoma were examined and compared with those in normal pancreas. Human pancreatic carcinoma contained increased levels (4-fold) of total GAGs. Particularly, this carcinoma is characterized by a 12-fold increase of hyaluronan (HA) and a 22-fold increase in chondroitin sulfate (CS) content. CS in pancreatic carcinoma exhibited an altered disaccharide composition which is associated with marked increase of non-sulfated and 6-sulfated disaccharides. Dermatan sulfate (DS) was also increased (1.5-fold) in carcinoma, whereas heparan sulfate (HS), the major GAG of normal pancreas, becomes the minor GAG in pancreatic carcinoma without significant changes in the content and in molecular size. In all cases, the galactosaminoglycans (GalGAGs, i.e. CS and DS) derived from pancreatic carcinomas were of lower molecular size compared to those from normal pancreas. The results in this study indicate, for the first time, that human pancreatic carcinoma is characterized by highly increased amounts of HA and of a structurally altered CS.
对人胰腺癌中存在的糖胺聚糖（GAGs）的数量和类型进行了检测，并与正常胰腺中的进行了比较。人胰腺癌中总GAGs水平升高（4倍）。特别是，这种癌的特征是透明质酸（HA）增加了12倍，硫酸软骨素（CS）含量增加了22倍。胰腺癌中的CS表现出二糖组成的改变，这与非硫酸化和6-硫酸化二糖的显著增加有关。硫酸皮肤素（DS）在癌中也增加了（1.5倍），而正常胰腺中的主要GAG硫酸乙酰肝素（HS）在胰腺癌中成为次要GAG，其含量和分子大小没有显著变化。在所有情况下，与正常胰腺来源的半乳糖胺聚糖（GalGAGs，即CS和DS）相比，胰腺癌来源的分子大小更低。本研究结果首次表明，人胰腺癌的特征是HA含量大幅增加以及CS结构改变。
