叉状DNA模板支持绕过在单链模板上阻断DNA合成的损伤进行复制。
Fork-like DNA templates support bypass replication of lesions that block DNA synthesis on single-stranded templates.
作者信息
Hoffmann J S, Pillaire M J, Lesca C, Burnouf D, Fuchs R P, Defais M, Villani G
机构信息
Institut de Pharmacologie et de Biologie Structurale, Centre National de la Recherche Scientifique, Unité Propre de Recherche 9062, Toulouse, France.
出版信息
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13766-9. doi: 10.1073/pnas.93.24.13766.
Abstract
DNA replication is an asymmetric process involving concurrent DNA synthesis on leading and lagging strands. Leading strand synthesis proceeds concomitantly with fork opening, whereas synthesis of the lagging strand essentially takes place on a single-stranded template. The effect of this duality on DNA damage processing by the cellular replication machinery was tested using eukaryotic cell extracts and model DNA substrates containing site-specific DNA adducts formed by the anticancer drug cisplatin or by the carcinogen N-2-acetylaminofluorene. Bypass of both lesions was observed only with fork-like substrates, whereas complete inhibition of DNA synthesis occurred on damaged single-stranded DNA substrates. These results suggest a role for additional accessory factors that permit DNA polymerases to bypass lesions when present in fork-like DNA.
摘要
DNA复制是一个不对称过程，涉及前导链和后随链上的同时DNA合成。前导链合成与复制叉打开同时进行，而后随链的合成基本上发生在单链模板上。利用真核细胞提取物和含有由抗癌药物顺铂或致癌物N-2-乙酰氨基芴形成的位点特异性DNA加合物的模型DNA底物，测试了这种双重性对细胞复制机制处理DNA损伤的影响。仅在叉状底物上观察到两种损伤的绕过，而在受损的单链DNA底物上发生了DNA合成的完全抑制。这些结果表明，当存在于叉状DNA中时，额外的辅助因子可使DNA聚合酶绕过损伤。
相似文献
1
Fork-like DNA templates support bypass replication of lesions that block DNA synthesis on single-stranded templates.叉状DNA模板支持绕过在单链模板上阻断DNA合成的损伤进行复制。
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13766-9. doi: 10.1073/pnas.93.24.13766.
2
Escherichia coli PriA helicase: fork binding orients the helicase to unwind the lagging strand side of arrested replication forks.大肠杆菌PriA解旋酶：叉形结构结合使解旋酶定向，以解开停滞复制叉的滞后链一侧。
J Mol Biol. 2001 Oct 5;312(5):935-47. doi: 10.1006/jmbi.2001.4930.
3
Differential replication of a single, UV-induced lesion in the leading or lagging strand by a human cell extract: fork uncoupling or gap formation.人类细胞提取物对前导链或后随链中单个紫外线诱导损伤的差异复制：叉解偶联或缺口形成。
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):11975-9. doi: 10.1073/pnas.92.26.11975.
4
DNA synthesis by CHO cell extracts on fork-like DNA templates containing the major cisplatin adduct requires a ligation step.在含有主要顺铂加合物的叉状DNA模板上，CHO细胞提取物进行DNA合成需要连接步骤。
Biochimie. 2000 Jan;82(1):41-9. doi: 10.1016/s0300-9084(00)00340-0.
5
Mutagenic replication in human cell extracts of DNA containing site-specific N-2-acetylaminofluorene adducts.含位点特异性 N-2-乙酰氨基芴加合物的 DNA 在人细胞提取物中的诱变复制。
Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7752-6. doi: 10.1073/pnas.91.16.7752.
6
Differential replication of a single N-2-acetylaminofluorene lesion in the leading or lagging strand DNA in a human cell extract.在人细胞提取物中，单个N-2-乙酰氨基芴损伤在DNA前导链或后随链中的差异复制。
J Biol Chem. 1997 Jun 13;272(24):15351-7. doi: 10.1074/jbc.272.24.15351.
7
N-2-aminofluorene and N-2 acetylaminofluorene adducts: the local sequence context of an adduct and its chemical structure determine its replication properties.N-2-氨基芴和N-2-乙酰氨基芴加合物：加合物的局部序列背景及其化学结构决定其复制特性。
J Mol Biol. 1995 Jun 23;249(5):903-13. doi: 10.1006/jmbi.1995.0347.
8
HMG1 protein inhibits the translesion synthesis of the major DNA cisplatin adduct by cell extracts.HMG1蛋白可抑制细胞提取物对主要DNA顺铂加合物的跨损伤合成。
J Mol Biol. 1997 Jul 25;270(4):539-43. doi: 10.1006/jmbi.1997.1143.
9
Uncoupling of leading- and lagging-strand DNA replication during lesion bypass in vivo.体内损伤绕过过程中前导链和后随链DNA复制的解偶联
Science. 2003 May 23;300(5623):1300-3. doi: 10.1126/science.1083964.
10
The application of a minicircle substrate in the study of the coordinated T4 DNA replication.一种微小环状底物在T4 DNA复制协同研究中的应用。
J Biol Chem. 2003 Dec 12;278(50):49828-38. doi: 10.1074/jbc.M307406200. Epub 2003 Sep 18.
引用本文的文献
1
High-Affinity Low-Capacity and Low-Affinity High-Capacity N-Acetyl-2-Aminofluorene (AAF) Macromolecular Binding Sites Are Revealed During the Growth Cycle of Adult Rat Hepatocytes in Primary Culture.在原代培养的成年大鼠肝细胞生长周期中，揭示了高亲和力低容量和低亲和力高容量 N-乙酰-2-氨基芴（AAF）大分子结合位点。
Toxicol Sci. 2018 May 1;163(1):35-44. doi: 10.1093/toxsci/kfy007.
2
Review of Cisplatin and Oxaliplatin in Current Immunogenic and Monoclonal Antibodies Perspective.从当前免疫原性和单克隆抗体角度对顺铂和奥沙利铂的综述
World J Oncol. 2014 Jun;5(3):97-108. doi: 10.14740/wjon830w. Epub 2014 Jun 25.
3
Review of Cisplatin and oxaliplatin in current immunogenic and monoclonal antibody treatments.顺铂和奥沙利铂在当前免疫原性及单克隆抗体治疗中的综述。
Oncol Rev. 2014 Sep 23;8(2):256. doi: 10.4081/oncol.2014.256.
4
Necrotizing lymphadenitis (NEL) is a systemic disease characterized by blastic transformation of CD8+ cells and apoptosis of CD4+ cells.坏死性淋巴结炎（NEL）是一种全身性疾病，其特征为 CD8+细胞的母细胞转化和 CD4+细胞的凋亡。
Virchows Arch. 2014 Jan;464(1):95-103. doi: 10.1007/s00428-013-1516-z. Epub 2013 Nov 30.
5
Transcription inhibition by platinum-DNA cross-links in live mammalian cells.铂-DNA 交联物在活哺乳动物细胞中的转录抑制作用。
J Am Chem Soc. 2010 Jun 2;132(21):7429-35. doi: 10.1021/ja101495v.
6
Meiosis-specific yeast Hop1 protein promotes synapsis of double-stranded DNA helices via the formation of guanine quartets.减数分裂特异性酵母Hop1蛋白通过形成鸟嘌呤四联体促进双链DNA螺旋的联会。
Nucleic Acids Res. 2004 Apr 28;32(8):2378-85. doi: 10.1093/nar/gkh559. Print 2004.
7
Impaired translesion synthesis in xeroderma pigmentosum variant extracts.着色性干皮病变异型提取物中跨损伤合成受损。
Mol Cell Biol. 1999 Mar;19(3):2206-11. doi: 10.1128/MCB.19.3.2206.
8
Abnormal, error-prone bypass of photoproducts by xeroderma pigmentosum variant cell extracts results in extreme strand bias for the kinds of mutations induced by UV light.着色性干皮病变异型细胞提取物对光产物进行异常且易出错的旁路修复，导致紫外线诱导的突变类型出现极端的链偏向性。
Mol Cell Biol. 1999 Jan;19(1):147-54. doi: 10.1128/MCB.19.1.147.
9
Overexpression of DNA polymerase beta in cell results in a mutator phenotype and a decreased sensitivity to anticancer drugs.细胞中DNA聚合酶β的过表达会导致突变表型以及对抗癌药物的敏感性降低。
Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12586-90. doi: 10.1073/pnas.95.21.12586.
10
Analysis of damage tolerance pathways in Saccharomyces cerevisiae: a requirement for Rev3 DNA polymerase in translesion synthesis.酿酒酵母中损伤耐受途径的分析：跨损伤合成中Rev3 DNA聚合酶的需求。
Mol Cell Biol. 1998 Feb;18(2):960-6. doi: 10.1128/MCB.18.2.960.
本文引用的文献
1
In vitro bypass replication of the cisplatin-d(GpG) lesion by calf thymus DNA polymerase beta and human immunodeficiency virus type I reverse transcriptase is highly mutagenic.通过小牛胸腺DNA聚合酶β和I型人类免疫缺陷病毒逆转录酶对顺铂-d(GpG)损伤进行体外旁路复制具有高度致突变性。
J Biol Chem. 1996 Jun 28;271(26):15386-92. doi: 10.1074/jbc.271.26.15386.
2
Strand specificity of mutagenic bypass replication of DNA containing psoralen monoadducts in a human cell extract.人细胞提取物中含补骨脂素单加合物的DNA诱变绕过复制的链特异性
Mol Cell Biol. 1996 May;16(5):2537-44. doi: 10.1128/MCB.16.5.2537.
3
Differential replication of a single, UV-induced lesion in the leading or lagging strand by a human cell extract: fork uncoupling or gap formation.人类细胞提取物对前导链或后随链中单个紫外线诱导损伤的差异复制：叉解偶联或缺口形成。
Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):11975-9. doi: 10.1073/pnas.92.26.11975.
4
Coupling of a replicative polymerase and helicase: a tau-DnaB interaction mediates rapid replication fork movement.复制性聚合酶与解旋酶的偶联：tau-DnaB相互作用介导快速复制叉移动。
Cell. 1996 Feb 23;84(4):643-50. doi: 10.1016/s0092-8674(00)81039-9.
5
DNA substrates for studying replication mechanisms: synthetic replication forks.用于研究复制机制的DNA底物：合成复制叉。
Methods Enzymol. 1995;262:457-66. doi: 10.1016/0076-6879(95)62037-0.
6
Replication and mutagenesis of UV-damaged DNA templates in human and monkey cell extracts.紫外线损伤的DNA模板在人和猴细胞提取物中的复制与诱变
Mol Cell Biol. 1993 Jan;13(1):533-42. doi: 10.1128/mcb.13.1.533-542.1993.
7
8
Greater susceptibility to mutations in lagging strand of DNA replication in Escherichia coli than in leading strand.大肠杆菌中DNA复制后随链比前导链对突变更敏感。
Science. 1993 Jul 30;261(5121):598-600. doi: 10.1126/science.8342022.
9
Use of single-turnover kinetics to study bulky adduct bypass by T7 DNA polymerase.利用单轮动力学研究T7 DNA聚合酶对大体积加合物的旁路作用。
Biochemistry. 1994 Jan 25;33(3):764-72. doi: 10.1021/bi00169a018.
10
Interaction of DNA polymerase and DNA helicase within the bacteriophage T4 DNA replication complex. Leading strand synthesis by the T4 DNA polymerase mutant A737V (tsL141) requires the T4 gene 59 helicase assembly protein.噬菌体T4 DNA复制复合物中DNA聚合酶与DNA解旋酶的相互作用。T4 DNA聚合酶突变体A737V（tsL141）进行前导链合成需要T4基因59解旋酶组装蛋白。
J Biol Chem. 1994 Jan 7;269(1):447-55.
Zotero 插件