Critchley H O, Kelly R W, Lea R G, Drudy T A, Jones R L, Baird D T
Department of Obstetrics and Gynaecology, University of Edinburgh, UK.
Hum Reprod. 1996 Oct;11(10):2257-62. doi: 10.1093/oxfordjournals.humrep.a019086.
Endometrial leukocyte subpopulations vary over the reproductive cycle, but no data exist on the mechanism regulating their recruitment into uterine tissue. This study has evaluated the role of progesterone in the recruitment of selected leukocyte populations in early pregnancy decidua. Decidua was collected from women in early pregnancy at the time of vacuum aspiration of the uterus 6, 12, 24 and 36 h after taking 200 mg mifepristone (RU486). Standard immunohistochemical techniques were employed to demonstrate the selected leukocyte populations in decidual tissue and these were analysed using imaged analysis. Fresh decidua was incubated in medium for 24 h and supernatants assayed for interleukin (IL-8) (neutrophil chemotactic factor) and MCP-1 (monocyte chemoattractant protein-1) content. Analysis of variance demonstrated a significant increase in tissue monocyte number in decidua 12-36 h after mifepristone administration. No significant changes in other leukocyte subpopulations were observed. Decidua IL-8 concentrations were significantly increased (P = 0.019) 6 h after mifepristone and decidual MCP-1 concentration rose (non-significant) and fell significantly (P = 0.029) between 6 and 12 h after mifepristone. Progesterone withdrawal may initiate a local cascade of events involving inflammatory mediators which in turn are responsible for the influx of monocytes. This influx may be essential in the process of shedding of endometrium or decidua since monocytes and neutrophils are important sources of proteases and collagenases. Furthermore, these cells are potential local sources of immunomodulatory cytokines.
子宫内膜白细胞亚群在生殖周期中会发生变化，但关于调节它们募集到子宫组织中的机制尚无数据。本研究评估了孕酮在早孕蜕膜中选定白细胞群体募集中的作用。在服用200mg米非司酮（RU486）后6、12、24和36小时，于子宫吸宫时从早孕妇女收集蜕膜。采用标准免疫组织化学技术在蜕膜组织中显示选定的白细胞群体，并使用图像分析对其进行分析。将新鲜蜕膜在培养基中孵育24小时，并检测上清液中白细胞介素（IL-8）（中性粒细胞趋化因子）和MCP-1（单核细胞趋化蛋白-1）的含量。方差分析表明，米非司酮给药后12 - 36小时，蜕膜中组织单核细胞数量显著增加。未观察到其他白细胞亚群有显著变化。米非司酮给药后6小时，蜕膜IL-8浓度显著升高（P = 0.019），蜕膜MCP-1浓度在米非司酮给药后6至12小时上升（无显著性）并显著下降（P = 0.029）。孕酮撤退可能引发一系列涉及炎症介质的局部事件，这些炎症介质进而导致单核细胞的流入。这种流入在子宫内膜或蜕膜脱落过程中可能至关重要，因为单核细胞和中性粒细胞是蛋白酶和胶原酶的重要来源。此外，这些细胞是免疫调节细胞因子的潜在局部来源。
