Calvert R J, Evans P A, Randerson J A, Jack A S, Morgan G J, Dixon M F
Department of Histopathology, General Infirmary at Leeds, U.K.
J Pathol. 1996 Sep;180(1):26-32. doi: 10.1002/(SICI)1096-9896(199609)180:1<26::AID-PATH681>3.0.CO;2-X.
The significance of the demonstration of a clonal B-cell population in gastric lymphoid infiltrates was investigated by analysis of immunoglobulin heavy chain (IgH) gene rearrangements using sensitive polymerase chain reactions, employing fluorescently labelled primers to target the FR3 and FR1 regions. Tissue blocks were studied showing different histological features (high-grade lymphoma, low-grade lymphoma, and chronic gastritis) from 12 gastrectomies for primary gastric lymphoma, together with blocks showing chronic gastritis from 13 cases of gastric adenocarcinoma and biopsies from 33 patients with active Helicobacter-associated chronic gastritis. Clonal IgH gene rearrangements were detected in lymphoma samples from eight of the gastrectomies for lymphoma (67 per cent). In four of these eight specimens, clonal rearrangements were also detectable in the samples showing only chronic gastritis. Three of 28 (11 per cent) informative biopsies showing active Helicobacter-associated chronic gastritis had detectable clonal populations. Clonal rearrangements were also demonstrated in two of eight (25 per cent) informative blocks showing chronic gastritis from eight gastrectomies for adenocarcinoma. It is concluded that the detection of a clonal population in a suspicious lymphoid infiltrate does not confirm the diagnosis of lymphoma, nor does the absence of such a population imply benignity.
通过使用敏感的聚合酶链反应分析免疫球蛋白重链（IgH）基因重排，采用荧光标记引物靶向FR3和FR1区域，研究了胃淋巴浸润中克隆性B细胞群体的显示意义。研究了来自12例原发性胃淋巴瘤胃切除术的具有不同组织学特征（高级别淋巴瘤、低级别淋巴瘤和慢性胃炎）的组织块，以及来自13例胃腺癌的显示慢性胃炎的组织块和33例活动性幽门螺杆菌相关性慢性胃炎患者的活检组织。在8例淋巴瘤胃切除术的淋巴瘤样本中检测到克隆性IgH基因重排（67%）。在这8个样本中的4个中，仅显示慢性胃炎的样本中也可检测到克隆性重排。28例显示活动性幽门螺杆菌相关性慢性胃炎的信息性活检中有3例（11%）检测到克隆性群体。在8例来自胃腺癌胃切除术的显示慢性胃炎的信息性组织块中的2例（25%）中也证实了克隆性重排。结论是，在可疑淋巴浸润中检测到克隆性群体并不能确诊淋巴瘤，没有这样的群体也不意味着良性。
