Morphopoulos G, Pearson M, Ryder W D, Howell A, Harris M
Department of Histopathology, Christie Hospital, Manchester, U.K.
J Pathol. 1996 Sep;180(1):44-9. doi: 10.1002/(SICI)1096-9896(199609)180:1<44::AID-PATH648>3.0.CO;2-C.
The importance of angiogenesis, assessed by tumour microvessel density, as a marker of survival was examined in 160 patients with infiltrating lobular carcinoma of the breast (ILC). The median follow-up was 5.1 years. Of these patients, 46 were node-negative, 59 were node-positive, and in 55 the pathological lymph node status was not known. Tumour sections were immunohistochemically stained with Factor VIII-related antibody. Microvessels were identified using previously recommended methodology and counted in three separate fields, selected from areas of highest vascularity, at x 200 magnification (field area = 0.785 mm2). Only the highest count was considered in the analysis. No association was found between microvessel density and age, menopausal status, tumour size, histological subtype, peritumoural vessel invasion, and lymph node involvement at presentation. There was no association between microvessel density and overall survival or relapse-free survival. These results suggest that microvessel density assessment, using currently recommended methods, is unlikely to be of prognostic value in ILC.
通过肿瘤微血管密度评估的血管生成作为生存标志物的重要性，在160例浸润性小叶乳腺癌（ILC）患者中进行了研究。中位随访时间为5.1年。这些患者中，46例为淋巴结阴性，59例为淋巴结阳性，55例患者的病理淋巴结状态未知。肿瘤切片用因子VIII相关抗体进行免疫组织化学染色。微血管采用先前推荐的方法进行识别，并在x 200放大倍数下（视野面积=0.785平方毫米），从血管最丰富的区域选取三个独立视野进行计数。分析中仅考虑最高计数。微血管密度与年龄、绝经状态、肿瘤大小、组织学亚型、肿瘤周围血管侵犯以及就诊时的淋巴结受累情况之间均未发现关联。微血管密度与总生存期或无复发生存期之间也无关联。这些结果表明，使用当前推荐的方法评估微血管密度，在ILC中不太可能具有预后价值。
