Nakashima M, Ito M, Ohtsuru A, Alipov G K, Matsuzaki S, Nakayama T, Yamashita S, Sekine I
Department of Pathology and Cell Physiology, Atomic Disease Institute, Nagasaki University School of Medicine, Nagasaki, Japan.
J Pathol. 1996 Sep;180(1):80-4. doi: 10.1002/(SICI)1096-9896(199609)180:1<80::AID-PATH617>3.0.CO;2-A.
The giant cell tumour of tendon sheath (GCTTS) is mainly composed of mononucleated stromal cells (SC) and multinucleated giant cells (GC), so-called osteoclast-like GC. It is thought that GC are derived from SC, but their precise relationship is not fully understood. Parathyroid hormone (PTH)-related peptide (PTHrP) is now considered to be a cytokine for cell differentiation, which may stimulate osteoclast-like cell formation in haematopoietic cells. Five cases of GCTTS were evaluated immunohistochemically, using a variety of antibodies against PTHrP, PTH/PTHrP receptor, KP-1 as a histiocytic phenotypic antigen, fibronectin as a fibroblastic phenotypic antigen, and proliferating cell nuclear antigen (PCNA). In situ hybridization and immunohistochemistry revealed that in all cases both SC and GC expressed PTHrP. PTH/PTHrP receptor was observed only in histiocytic SC and GC, but not in fibroblastic SC. Almost all GC showed histiocytic features. PCNA immunoreactivity was detected only in the nuclei of SC, and not in GC. Moreover, SC with PTH/PTHrP receptor immunoreactivity were negative for PCNA. These results suggest that GC are derived from histiocytic SC expressing PTH/PTHrP receptor and losing proliferative activity in the process of transition from mononuclear to multinucleated. PTHrP produced by SC and GC may be involved in the formation of osteoclast-like cells in GCTTS by acting in an autocrine/paracrine fashion.
腱鞘巨细胞瘤（GCTTS）主要由单核基质细胞（SC）和多核巨细胞（GC）组成，即所谓的破骨细胞样GC。一般认为GC来源于SC，但其确切关系尚未完全明确。甲状旁腺激素（PTH）相关肽（PTHrP）目前被认为是一种细胞分化细胞因子，可能刺激造血细胞中破骨细胞样细胞的形成。使用多种针对PTHrP、PTH/PTHrP受体、作为组织细胞表型抗原的KP-1、作为成纤维细胞表型抗原的纤连蛋白以及增殖细胞核抗原（PCNA）的抗体，对5例GCTTS进行了免疫组织化学评估。原位杂交和免疫组织化学显示，在所有病例中，SC和GC均表达PTHrP。仅在组织细胞性SC和GC中观察到PTH/PTHrP受体，而成纤维细胞性SC中未观察到。几乎所有GC均表现出组织细胞特征。PCNA免疫反应仅在SC细胞核中检测到，而在GC中未检测到。此外，具有PTH/PTHrP受体免疫反应性的SC对PCNA呈阴性。这些结果表明，GC来源于表达PTH/PTHrP受体并在从单核向多核转变过程中失去增殖活性的组织细胞性SC。SC和GC产生的PTHrP可能通过自分泌/旁分泌方式参与GCTTS中破骨细胞样细胞的形成。
