Healey J F, Lubin I M, Lollar P
Department of Medicine, Emory University, Atlanta GA 30322, USA.
Blood. 1996 Dec 1;88(11):4209-14.
The cDNA corresponding to 137 bp of the 5' untranslated region, the signal peptide, and the A1, A3, C1, and C2 domains of porcine factor VIII (fVIII) have been cloned and sequenced. Along with previously determined sequences of the porcine fVIII B domain and the A2 domain, this completes the sequence determination of the cDNA corresponding to the translated protein. Alignments of the derived amino acid sequence of porcine fVIII with human and murine fVIII indicate that the A1, A2, A3, C1, and C2 domains are more conserved than the B domains or the proteolytic cleavage peptides corresponding to residues 337-372 and 1649-1689. The knowledge of the porcine fVIII cDNA may be useful to understand functional and immunological differences between human and porcine fVIII and may lead to improved fVIII replacement products for hemophilia. A patients through the development of recombinant porcine fVIII or hybrid human/porcine fVIII derivatives.
已克隆并测序了与猪因子VIII（fVIII）5'非翻译区137 bp、信号肽以及A1、A3、C1和C2结构域相对应的cDNA。连同先前确定的猪fVIII B结构域和A2结构域的序列，这完成了与翻译后蛋白质相对应的cDNA的序列测定。猪fVIII推导的氨基酸序列与人及小鼠fVIII的比对表明，A1、A2、A3、C1和C2结构域比B结构域或对应于337 - 372位和1649 - 1689位残基的蛋白水解切割肽更保守。猪fVIII cDNA的知识可能有助于理解人fVIII和猪fVIII之间的功能和免疫学差异，并可能通过开发重组猪fVIII或人/猪fVIII杂交衍生物为血友病A患者带来改良的fVIII替代产品。
