Chu K U, Ishizuka J, Battey J F, Uchida T, Beauchamp R D, Townsend C M, Thompson J C
Department of Surgery and Biostatistics, University of Texas Medical Branch, Galveston 77555, USA.
Dig Dis Sci. 1996 Nov;41(11):2180-6. doi: 10.1007/BF02071398.
The growth of the human gastrinoma model (PT) in athymic nude mice is stimulated by bombesin (BBS), an amphibian peptide homologous to both human gastrin-releasing peptide (GRP) and neuromedin B (NMB). The mechanism is not known, and a potent and specific GRP-R antagonist BIM26226, which has low affinity for NMB-R, was used in vivo in athymic nude mice bearing gastrinoma subcutaneously. Both the BBS and BIM26226 stimulated the growth of PT, and the growth stimulation was even greater when given together. RT-PCR study of gastrinoma revealed the presence of both GRP-R and NMB-R mRNA, but much more abundant NMB-R mRNA. We conclude that BBS-stimulated growth of gastrinoma involves both GRP-R and NMB-R, and our findings suggest that GRP-R mediates negative and NMB-R produces positive growth effects on gastrinoma.
蛙皮素（BBS）可刺激人胃泌素瘤模型（PT）在无胸腺裸鼠体内生长，蛙皮素是一种两栖类肽，与人胃泌素释放肽（GRP）和神经介素B（NMB）同源。其机制尚不清楚，对NMB-R亲和力低的强效特异性GRP-R拮抗剂BIM26226被用于皮下接种胃泌素瘤的无胸腺裸鼠体内实验。BBS和BIM26226均刺激了PT的生长，二者共同给药时生长刺激作用更强。胃泌素瘤的逆转录聚合酶链反应（RT-PCR）研究显示，同时存在GRP-R和NMB-R的信使核糖核酸（mRNA），但NMB-R的mRNA含量更为丰富。我们得出结论，BBS刺激胃泌素瘤生长涉及GRP-R和NMB-R二者，我们的研究结果表明，GRP-R介导负性生长效应，而NMB-R对胃泌素瘤产生正性生长效应。
