Claros M G, Vincens P
Laboratoire de Génétique Moléculaire, CNRS URA 1302, Ecole Normale Supérieure, Paris, France.
Eur J Biochem. 1996 Nov 1;241(3):779-86. doi: 10.1111/j.1432-1033.1996.00779.x.
Most of the proteins that are used in mitochondria are imported through the double membrane of the organelle. The information that guides the protein to mitochondria is contained in its sequence and structure, although no direct evidence can be obtained. In this article, discriminant analysis has been performed with 47 parameters and a large set of mitochondrial proteins extracted from the SwissProt database. A computational method that facilitates the analysis and objective prediction of mitochondrially imported proteins has been developed. If only the amino acid sequence is considered, 75-97% of the mitochondrial proteins studied have been predicted to be imported into mitochondria. Moreover, the existence of mitochondrial-targeting sequences is predicted in 76-94% of the analyzed mitochondrial precursor proteins. As a practical application, the number of unknown yeast open reading frames that might be mitochondrial proteins has been predicted, which revealed that many of them are clustered.
大多数在线粒体中发挥作用的蛋白质都是通过该细胞器的双层膜导入的。尽管无法获得直接证据，但引导蛋白质进入线粒体的信息包含在其序列和结构中。在本文中，利用从瑞士蛋白质数据库中提取的47个参数和大量线粒体蛋白质进行了判别分析。已开发出一种有助于分析和客观预测线粒体导入蛋白质的计算方法。如果仅考虑氨基酸序列，所研究的线粒体蛋白质中有75% - 97%被预测可导入线粒体。此外，在76% - 94%的分析线粒体前体蛋白中预测存在线粒体靶向序列。作为实际应用，已预测出可能是线粒体蛋白质的未知酵母开放阅读框的数量，结果表明其中许多是成簇的。
