Marsh A E, Barr B C, Madigan J, Lakritz J, Nordhausen R, Conrad P A
Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis 95616-8745, USA.
J Am Vet Med Assoc. 1996 Dec 1;209(11):1907-13.
Neosporosis was diagnosed in an 11-year-old Quarter Horse gelding with clinical signs and diagnostic test results compatible with equine protozoal myeloencephalitis (EPM). Presumptive postmortem diagnosis of EPM attributable to Sarcocystis neurona infection is generally made on the basis of detecting an antibody titer to S neurona in the CSF or characteristic histologic lesions, even when parasites have not been specifically identified. Neosporosis was confirmed in the horse described here by use of immunohistochemical examination, in vitro culturing, and ultrastructural and molecular characterization of parasites from infected tissues. Antibody testing of serum and CSF samples indicated that Neospora-specific anti-bodies can react with S neurona proteins on western blot analysis. The confirmation that neosporosis in horses can mimic EPM emphasizes the need to broaden the etiologic definition of EPM beyond infections exclusively attributable to S neurona.
在一匹11岁的夸特马种公马中诊断出了新孢子虫病，其临床症状和诊断测试结果与马原虫性脑脊髓炎（EPM）相符。即使在未明确鉴定出寄生虫的情况下，通常根据检测脑脊液中针对神经肉孢子虫的抗体滴度或特征性组织学病变，对归因于神经肉孢子虫感染的EPM进行死后推定诊断。在此描述的马匹中，通过免疫组织化学检查、体外培养以及对感染组织中的寄生虫进行超微结构和分子特征分析，确诊了新孢子虫病。血清和脑脊液样本的抗体检测表明，在蛋白质印迹分析中，新孢子虫特异性抗体可与神经肉孢子虫蛋白发生反应。马新孢子虫病可模拟EPM这一事实的确认强调了有必要将EPM的病因定义扩大到不仅仅局限于神经肉孢子虫感染。
