Tomizawa Y, Noishiki Y, Okoshi T, Nishida H, Endo M, Koyanagi H
Department of Cardiovascular Surgery, Heart Institute of Japan, Tokyo Women's Medical College, Japan.
ASAIO J. 1996 Sep-Oct;42(5):M698-702. doi: 10.1097/00002480-199609000-00077.
Angiogenesis by basic fibroblast growth factor (bFGF) is important for endothelialization in vascular prostheses and is significant from shortly after implantation. Canine adipose tissue was resected, minced into fragments, and suspended. This mixture was sieved through the wall of a fabric vascular prosthesis. Tissue-fragmented grafts (TF-grafts, 6 mm internal diameter, 6 cm long) were implanted into the abdominal aortae of four dogs, and four preclotted grafts were used as control subjects. Grafts were removed at 1-7 days after implantation. Removed grafts were evaluated microscopically, immunohistologically, and by scanning electron microscope. At day 1, a thrombus layer was on the TF-graft lumen. At day 3, cell proliferation and migration were observed. At day 5, endothelial-like cells were extending onto the luminal thrombus. Cell proliferation around the fragments was active, and those cells were bFGF positive. In the control subjects, at day 7, the perigraft tissue was bFGF positive, whereas no endothelialization on the lumen or no capillary infiltration into the graft wall was observed. Furthermore, bFGF was negative in the sites of thrombus and infection. These results demonstrate that endogenous bFGF is important for endothelialization due to angiogenesis in fabric vascular prostheses, whereas thrombus and infection might have a negative effect.
碱性成纤维细胞生长因子（bFGF）介导的血管生成对血管假体的内皮化很重要，且在植入后不久就很显著。切除犬的脂肪组织，切碎成碎片并悬浮。将该混合物通过织物血管假体的壁进行筛分。将组织碎片化移植物（TF移植物，内径6mm，长6cm）植入四只犬的腹主动脉，四只预凝血移植物用作对照。在植入后1 - 7天取出移植物。对取出的移植物进行显微镜、免疫组织学和扫描电子显微镜评估。在第1天，TF移植物管腔内有血栓层。在第3天，观察到细胞增殖和迁移。在第5天，内皮样细胞延伸到腔内血栓上。碎片周围的细胞增殖活跃，且这些细胞bFGF呈阳性。在对照组中，在第7天，移植物周围组织bFGF呈阳性，而管腔内未观察到内皮化，移植物壁内也没有毛细血管浸润。此外，血栓和感染部位bFGF呈阴性。这些结果表明，内源性bFGF对织物血管假体中血管生成导致的内皮化很重要，而血栓和感染可能具有负面影响。
