大鼠离体生精小管液体分泌速率的体外测量：代谢抑制剂和离子的作用
In vitro measurement of rate of fluid secretion in rat isolated seminiferous tubules: effects of metabolic inhibitors and ions.
作者信息
Cheung Y M, Hwang J C, Wong P Y
出版信息
J Physiol. 1977 Jul;269(1):1-15. doi: 10.1113/jphysiol.1977.sp011889.
Abstract
- An in vitro technique for measuring secretory rate in rat isolated seminiferous tubules is described. 2. The basal rate of fluid secretion was 0-44+/-0-06 nl. cm-1 min-1 (S.E.) (n=21). The rate was found to be inhibited by cooling, addition of metabolic inhibitor 2,4-dinitrophenol (2-5 x 10(-4) M) and removal of glucose from the incubating solution. This indicates that fluid secretion in isolated rat seminiferous tubules is an energy dependent process. 3. Removal of K+ from the incubating medium inhibited the secretory rate in the isolated seminiferous tubules, whereas a fivefold increase in [K+]0 to 23-5 mM had no effect. The secretory rate was also unaffected by the absence of Cl- in the peritubular fluid. 4. Removal of Ca2+ from the peritubular medium caused a rise in the secretory rate. 5. Ouabain (10(-3) M) and acetazolamide (4 x 10(-5) M) caused a fall in the rate of fluid secretion in isolated seminiferous tubules. 6. These results are discussed in relation to the nature of the ionic secretion produced in the tubules.
摘要
- 本文描述了一种用于测量大鼠离体生精小管分泌率的体外技术。2. 液体分泌的基础速率为0 - 44±0 - 06 nl·cm⁻¹·min⁻¹（标准误）（n = 21）。发现该速率会受到冷却、添加代谢抑制剂2,4 - 二硝基苯酚（2.5×10⁻⁴ M）以及从孵育溶液中去除葡萄糖的抑制。这表明大鼠离体生精小管中的液体分泌是一个能量依赖过程。3. 从孵育培养基中去除K⁺会抑制离体生精小管的分泌率，而将[K⁺]₀增加五倍至23.5 mM则没有影响。分泌率也不受管周液中Cl⁻缺失的影响。4. 从管周培养基中去除Ca²⁺会导致分泌率升高。5. 哇巴因（10⁻³ M）和乙酰唑胺（4×10⁻⁵ M）会导致离体生精小管中液体分泌率下降。6. 结合小管中产生的离子分泌的性质对这些结果进行了讨论。
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In vitro measurement of rate of fluid secretion in rat isolated seminiferous tubules: effects of metabolic inhibitors and ions.大鼠离体生精小管液体分泌速率的体外测量：代谢抑制剂和离子的作用
J Physiol. 1977 Jul;269(1):1-15. doi: 10.1113/jphysiol.1977.sp011889.
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