霍乱弧菌外膜蛋白OmpU编码基因的克隆与特性分析
Cloning and characterization of the gene encoding the OmpU outer membrane protein of Vibrio cholerae.
作者信息
Sperandio V, Bailey C, Girón J A, DiRita V J, Silveira W D, Vettore A L, Kaper J B
机构信息
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore 21201, USA.
出版信息
Infect Immun. 1996 Dec;64(12):5406-9. doi: 10.1128/iai.64.12.5406-5409.1996.
Abstract
The OmpU outer membrane protein is a member of the ToxR regulon of Vibrio cholerae and has recently been shown to be a potential adherence factor for this species. Using PCR and degenerate oligonucleotide primers based on internal peptide sequences of purified OmpU, we have cloned and sequenced the gene encoding OmpU. The ompU gene is predicted to encode a 36,646-molecular-weight protein which is present in both cholera toxin-positive and -negative V. cholerae O1 and O139 strains.
摘要
外膜蛋白OmpU是霍乱弧菌ToxR调节子的成员，最近已被证明是该菌潜在的黏附因子。利用基于纯化的OmpU内部肽段序列的PCR和简并寡核苷酸引物，我们克隆并测序了编码OmpU的基因。ompU基因预计编码一种分子量为36,646的蛋白质，该蛋白存在于霍乱毒素阳性和阴性的霍乱弧菌O1和O139菌株中。
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