具有降低的ADP-核糖基化活性或无活性的大肠杆菌热不稳定肠毒素突变体保留了天然全毒素的免疫原性。
Mutants of the Escherichia coli heat-labile enterotoxin with reduced ADP-ribosylation activity or no activity retain the immunogenic properties of the native holotoxin.
作者信息
de Haan L, Verweij W R, Feil I K, Lijnema T H, Hol W G, Agsteribbe E, Wilschut J
机构信息
Department of Physiological Chemistry, Groningen Institute for Drug Studies, University of Groningen, The Netherlands.
出版信息
Infect Immun. 1996 Dec;64(12):5413-6. doi: 10.1128/iai.64.12.5413-5416.1996.
Abstract
The Escherichia coli heat-labile enterotoxin (LT) is a potent inducer of mucosal immune responses. In a previous study (L. DeHaan, W. R. Verweij, M. Holtrop, E. Agsteribbe, and J. Wilschut, Vaccine 14:620-626, 1996), we have shown that efficient induction of an LTB-specific mucosal immune response by LT requires the presence of the LTA chain, suggesting a possible role of the enzymatic activity of LTA in the induction of these responses. In the present study, we generated LT mutants with altered ADP-ribosylation activities and evaluated their immunogenicity upon intranasal administration to mice. The results demonstrate that the mucosal immunogenicity of LT is not dependent on its ADP-ribosylation activity.
摘要
大肠杆菌热不稳定肠毒素（LT）是黏膜免疫反应的强效诱导剂。在之前的一项研究中（L. 德哈恩、W. R. 韦尔韦伊、M. 霍尔托普、E. 阿格斯特里布和J. 威尔舒特，《疫苗》14:620 - 626，1996年），我们已经表明，LT高效诱导LTB特异性黏膜免疫反应需要LTA链的存在，这表明LTA的酶活性在诱导这些反应中可能发挥作用。在本研究中，我们构建了具有改变的ADP - 核糖基化活性的LT突变体，并评估了它们经鼻内给予小鼠后的免疫原性。结果表明，LT的黏膜免疫原性不依赖于其ADP - 核糖基化活性。
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