Xavier F, Yu M, McNeill J R
Department of Pharmacology, Faculty of Medicine, University of Saskatchewan, Saskatoon, Canada.
J Cardiovasc Pharmacol. 1996 Nov;28(5):605-10. doi: 10.1097/00005344-199611000-00001.
Splenic blood-volume changes to norepinephrine were determined in the anesthetized cat by recording the integral of the difference between splenic arterial flow and splenic venous flow (flow-differential technique) with ultrasonic transit-time flowmeters, and the changes recorded with this technique were compared with those recorded with an independent technique, the gravimetric method. Norepinephrine (0.003-0.3 microgram/min/kg of body weight) induced dose-related decreases in splenic volume, and the values recorded by the flow-differential technique were not significantly different from those recorded with the gravimetric technique. The values recorded by the two methods were highly correlated (r = 0.86). The slope of the line plotting the relation between the two methods was close to unity (0.97 +/- 0.12), and the intercept of the line was close to zero (-0.46 +/- 1.6 ml). The results suggest that the recording of the flow differential between splenic arterial flow and splenic venous flow with ultrasonic transit time flowmeters is a valid method for the recording of splenic blood-volume changes to vasoactive agents.
在麻醉猫身上，通过使用超声渡越时间流量计记录脾动脉血流与脾静脉血流之差的积分（流量差技术）来测定脾脏血容量对去甲肾上腺素的变化，并将该技术记录的变化与另一种独立技术——重量法记录的变化进行比较。去甲肾上腺素（0.003 - 0.3微克/分钟/千克体重）引起脾脏体积呈剂量相关的减小，流量差技术记录的值与重量法记录的值无显著差异。两种方法记录的值高度相关（r = 0.86）。绘制两种方法关系的直线斜率接近1（0.97±0.12），直线截距接近零（-0.46±1.6毫升）。结果表明，用超声渡越时间流量计记录脾动脉血流与脾静脉血流之间的流量差是记录血管活性药物引起的脾脏血容量变化的有效方法。
