Misiewicz B, Zelazowska E, Raybourne R B, Cizza G, Sternberg E M
Clinical Neuroendocrinology Branch, National Institute of Mental Health, Bethesda, MD 20892, USA.
Neuroimmunomodulation. 1996 Mar-Jun;3(2-3):93-101. doi: 10.1159/000097233.
We studied the inflammatory responses of LEW/N and F344/N inbred rat strains after peripheral injection of carrageenan. The inflammatory responses were assessed in terms of volume, relative and total white blood cell counts of the exudates. Moreover, in both strains, blood CD4, CD8, CD25, naive CD4 (CD4/CD45RC) cell and B (CD45R) cell counts and plasma corticosterone levels, constituents of systemic inflammatory responses to carrageenan were evaluated. In general, LEW/N rats are highly responsive to challenge with carrageenan, whereas F344 rats are not. The strong local inflammatory responses to carrageenan are primarily exhibited by female LEW/N rats. The intensity of local inflammatory responses of LEW/N rats changes with the rat age, the highest exhibited by LEW/N rats up to 3 months of age, thereafter the carrageenan-induced inflammatory responses decline. Our results indicate that peripheral injection of carrageenan induces strong systemic immune component. After carrageenan injection, increases in CD8 and naive CD4 blood lymphocytes are seen. Although the carrageenan challenge does not change CD4 blood lymphocytes in both LEW/N and F344/N rat strains, LEW/N rats exhibit higher levels of CD4 cells than F344/N rats. Additionally, LEW/N rats demonstrated lower levels of B cells and higher naive CD4 lymphocytes. Carrageenan challenges induce significant increases in plasma corticosterone response in F344/N rats, as well as increases in LEW/N rats 1 h after injection. Our data stress the importance of rat age and gender in experiments studying inflammatory responses.
我们研究了在向LEW/N和F344/N近交系大鼠外周注射角叉菜胶后它们的炎症反应。根据渗出物的体积、相对白细胞计数和总白细胞计数来评估炎症反应。此外，在这两个品系中，还评估了血液中CD4、CD8、CD25、初始CD4（CD4/CD45RC）细胞和B（CD45R）细胞计数以及血浆皮质酮水平，这些都是对角叉菜胶全身性炎症反应的组成部分。一般来说，LEW/N大鼠对角叉菜胶刺激反应强烈，而F344大鼠则不然。对角叉菜胶强烈的局部炎症反应主要由雌性LEW/N大鼠表现出来。LEW/N大鼠局部炎症反应的强度随大鼠年龄而变化，3个月龄以下的LEW/N大鼠表现最为强烈，此后角叉菜胶诱导的炎症反应逐渐下降。我们的结果表明，外周注射角叉菜胶会诱导强烈的全身免疫成分。注射角叉菜胶后，可见血液中CD8和初始CD4淋巴细胞增加。虽然角叉菜胶刺激在LEW/N和F344/N大鼠品系中均未改变血液中的CD4淋巴细胞，但LEW/N大鼠的CD4细胞水平高于F344/N大鼠。此外，LEW/N大鼠的B细胞水平较低，初始CD4淋巴细胞水平较高。角叉菜胶刺激会使F344/N大鼠的血浆皮质酮反应显著增加，LEW/N大鼠在注射后1小时也会增加。我们的数据强调了大鼠年龄和性别在研究炎症反应实验中的重要性。
