Röijer E, Kas K, Klawitz I, Bullerdiek J, Van de Ven W, Stenman G
Department of Pathology, Göteborg University, Sweden.
Genes Chromosomes Cancer. 1996 Nov;17(3):166-71. doi: 10.1002/(SICI)1098-2264(199611)17:3<166::AID-GCC4>3.0.CO;2-2.
A subgroup of pleomorphic adenomas of the salivary glands is characterized by translocations involving chromosome 8, with consistent breakpoints at 8q12. As part of a positional cloning effort to isolate the gene(s) affected by these translocations we now report the mapping of the 8q12 breakpoint in two primary pleomorphic adenomas with the recurrent t(3;8)(p21;q12). Yeast artificial chromosome (YAC) clones corresponding to eight different loci in 8q11-12 were isolated and mapped by fluorescence in situ hybridization (FISH). The t(3;8) breakpoint was mapped within a 1 Mb region flanked by MOS proximally and by the genetic marker D8S166 distally. One YAC within this region was shown to span the t(3;8) breakpoint in two tumors. This YAC will provide an excellent tool for isolating the gene(s) at the breakpoint(s) in adenomas with t(3;8).
涎腺多形性腺瘤的一个亚组的特征是涉及8号染色体的易位，在8q12处有一致的断点。作为定位克隆以分离受这些易位影响的基因的一部分工作，我们现在报告在两例具有复发的t(3;8)(p21;q12)的原发性多形性腺瘤中8q12断点的定位。分离出与8q11-12中八个不同位点相对应的酵母人工染色体(YAC)克隆，并通过荧光原位杂交(FISH)进行定位。t(3;8)断点定位于一个1 Mb的区域内，近端侧翼为MOS，远端侧翼为遗传标记D8S166。该区域内的一个YAC显示在两个肿瘤中跨越t(3;8)断点。这个YAC将为分离具有t(3;8)的腺瘤断点处的基因提供一个极好的工具。
