Gupta R K, Roy R, Dev R, Husain M, Poptani H, Pandey R, Kishore J, Bhaduri A P
Department of Radiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Magn Reson Med. 1996 Dec;36(6):829-33. doi: 10.1002/mrm.1910360605.
In vivo, ex vivo, and in vitro proton magnetic resonance spectroscopy was performed in 12 patients with intracranial tuberculomas with an aim of detecting the biochemical constituents of Mycobacterium tuberculosis in a granuloma. One dimensional (1D) single pulse and spin-echo sequences and 2D correlative spectroscopy were used for the ex vivo study to confirm the resonances seen on in vivo study. Spectroscopic studies of the perchloric acid and lipid extract of granuloma and M. tuberculosis were performed to look for similarity of resonance. In vivo study showed the presence of lipids at 0.9, 1.3, 2.0, 2.8 ppm, and phosphoserine at 3.7 ppm. All these resonances were confirmed on ex vivo study. In addition, distinct resonances of serine and phenolic lipids were seen on ex vivo and in vitro study of tuberculous granuloma, which have not been observed in other intracranial tumors. Lipid extract of granuloma and M. tuberculosis showed phenolic lipids at 7.1 and 7.4 ppm, a constituent of the cell wall of the bacteria in a tuberculoma. It appears that it may be possible to finger print the biochemicals of the cell wall of M. tuberculosis in a tuberculous granuloma and thus may help in detection and diagnosis of such lesions.
对12例颅内结核瘤患者进行了体内、离体和体外质子磁共振波谱分析，目的是检测肉芽肿中结核分枝杆菌的生化成分。离体研究采用一维（1D）单脉冲和自旋回波序列以及二维相关波谱，以确认体内研究中观察到的共振信号。对肉芽肿和结核分枝杆菌的高氯酸提取物及脂质提取物进行了波谱研究，以寻找共振信号的相似性。体内研究显示在0.9、1.3、2.0、2.8 ppm处存在脂质，在3.7 ppm处存在磷酸丝氨酸。所有这些共振信号在离体研究中均得到证实。此外，在结核性肉芽肿的离体和体外研究中还观察到丝氨酸和酚类脂质的独特共振信号，这在其他颅内肿瘤中尚未观察到。肉芽肿和结核分枝杆菌的脂质提取物在7.1和7.4 ppm处显示出酚类脂质，这是结核瘤中细菌细胞壁的一种成分。看来有可能对结核性肉芽肿中结核分枝杆菌细胞壁的生化物质进行指纹识别，从而有助于此类病变的检测和诊断。
