Neidhart M, Pataki F, Michel B A, Fehr K
Department of Rheumatology, Zurich University Hospital, Zurich.
Schweiz Med Wochenschr. 1996 Nov 9;126(45):1922-5.
We investigated whether, in systemic lupus erythematosus (SLE), the CD45 isoforms expression on peripheral blood T-lymphocytes (T-PBL) is related to the auto-immune processes and hematological manifestations. The CD45RA/RO patterns of CD4+ and CD8 bright+ T-PBL were determined by three-colour flow cytometry. The serum levels of anti-nuclear (ANA), anti-double stranded DNA (ds DNA) and anti-cardiolipin (CL) autoantibodies were quantified by ELISA. The hematological parameters were routinely assessed. 72% of SLE patients (n = 29) had reduced lymphocyte counts, which correlated with more severe physician's assessment of disease activity, increased ANA and anti-ds DNA auto-antibodies. The lymphopenia preferentially affected the CD4+ T-PBL and, among them, the "naive" CD45RA+, RO- cells. Thus, compared with healthy women (n = 29), SLE patients had less naive and more "transient" CD45RA+, RO+ cells among CD4+ T-PBL. Meanwhile, on average, the CD45 isoforms expression on CD8+ T-PBL was unchanged. Interestingly, in 3 patients who were repeatedly evaluated, increases of transient CD8+ T-PBL paralleled the elevation of anti-ds DNA. In addition, high anti-CL was associated with more transient CD4+ and CD8+ T-PBL. The loss of naive and increase of transient CD8+ T-PBL was associated with increased disease activity and possibly hemolytic anemia. Thus, in SLE, the enhanced phenotypic switch from naive CD45RA+, RO- to "memory" CD45RO+ T-PBL patterns paralleled the auto-immune processes characteristic of this disease.
我们研究了在系统性红斑狼疮（SLE）中，外周血T淋巴细胞（T - PBL）上CD45异构体的表达是否与自身免疫过程及血液学表现相关。通过三色流式细胞术测定CD4⁺和CD8⁺亮⁺T - PBL的CD45RA/RO模式。采用酶联免疫吸附测定法（ELISA）定量检测血清中抗核抗体（ANA）、抗双链DNA（ds DNA）和抗心磷脂（CL）自身抗体水平。常规评估血液学参数。72%的SLE患者（n = 29）淋巴细胞计数减少，这与医生对疾病活动度更严重的评估、ANA和抗ds DNA自身抗体增加相关。淋巴细胞减少主要影响CD4⁺T - PBL，其中又以“初始”的CD45RA⁺、RO⁻细胞为主。因此，与健康女性（n = 29）相比，SLE患者CD4⁺T - PBL中初始细胞较少，而“短暂”的CD45RA⁺、RO⁺细胞较多。同时，平均而言，CD8⁺T - PBL上CD45异构体的表达没有变化。有趣的是，在3例接受反复评估的患者中，短暂性CD8⁺T - PBL的增加与抗ds DNA的升高平行。此外，高抗CL水平与更多短暂性CD4⁺和CD8⁺T - PBL相关。初始CD8⁺T - PBL的减少和短暂性CD8⁺T - PBL的增加与疾病活动度增加以及可能的溶血性贫血相关。因此，在SLE中，从初始CD45RA⁺、RO⁻到“记忆”CD45RO⁺T - PBL模式的表型转换增强与该疾病的自身免疫过程特征平行。
