Zabel L T, Neuer A, Manncke B
Department of Medical Microbiology, Tübingen, Germany.
Zentralbl Bakteriol. 1996 Sep;285(1):35-43. doi: 10.1016/s0934-8840(96)80020-x.
The matrix protein, fibronectin, which is detectable in various tissues, when present in the vaginal fluid of women in labour, indicates the rupture of membranes. It is known that many bacteria adhere to fibronectin, thus establishing a first step of infection. In women in labour, group B streptococci are common agents of chorioamnionitis. For group B streptococci, unspecific adherence mechanisms like negative net charge and hydrophobic interactions have already been discussed in literature. In the present study, group B streptococci isolates from 57 patients with premature rupture of membranes were studied for fibronectin binding activities, using a particle agglutination assay and for cell surface hydrophobicity, by testing adhesion to hydrocarbons. Particle agglutination assays and adhesion assays were done with strains grown on blood-containing media and media without blood. Fibronectin binding was shown to be present in 14 and 11 out of 57 isolates grown on Mueller-Hinton and Tryptic Soy agar, respectively. When the strains were grown on blood-containing media, fibronectin-binding was found to be concomitant with decreased hydrophobicity. According to the results obtained in a total of 57 strains, cell surface hydrophobicity is an unspecific adhesion factor in group B streptococci. Fibronectin binding seems to be an additional adherence factor in some of the strains and may be assumed to play a major role in establishing infectious processes.
基质蛋白纤连蛋白可在多种组织中检测到，当它存在于临产妇的阴道分泌物中时，表明胎膜已破裂。已知许多细菌会黏附于纤连蛋白，从而开启感染的第一步。在临产妇中，B族链球菌是绒毛膜羊膜炎的常见病原体。对于B族链球菌，文献中已讨论过诸如负净电荷和疏水相互作用等非特异性黏附机制。在本研究中，使用颗粒凝集试验研究了从57例胎膜早破患者中分离出的B族链球菌的纤连蛋白结合活性，并通过测试对碳氢化合物的黏附来研究细胞表面疏水性。颗粒凝集试验和黏附试验分别在含血培养基和无血培养基上培养的菌株上进行。在Mueller-Hinton琼脂和胰蛋白胨大豆琼脂上培养的57株分离株中，分别有14株和11株显示出纤连蛋白结合。当菌株在含血培养基上生长时，发现纤连蛋白结合与疏水性降低相关。根据总共57株菌株获得的结果，细胞表面疏水性是B族链球菌中的一种非特异性黏附因子。纤连蛋白结合似乎是某些菌株中的另一种黏附因子，并且可能在建立感染过程中起主要作用。
