Lewin A, Strauch E, Hertwig S, Hoffmann B, Nattermann H, Appel B
Robert Koch-Institut, Fachbereich Genetik und Gentechnik, Berlin, Germany.
Zentralbl Bakteriol. 1996 Sep;285(1):52-63. doi: 10.1016/s0934-8840(96)80022-3.
The plasmid content of apathogenic Y. enterocolitica biovar 1A strains was determined and the plasmids were compared with the virulence plasmid of a pathogenic Y. enterocolitica strain. About 38% of the selected biovar 1A strains contained plasmids of different sizes ranging from 2.7 kb to more than 70 kb. Some of the larger plasmids had a size similar to that of the virulence plasmid of a pathogenic reference strain. The restriction patterns of these plasmids were different from the restriction pattern of the virulence plasmid of the pathogenic reference strain. Differences were also observed in hybridization studies with the virulence plasmid. The plasmids from 15 out of 16 biovar 1A strains showed no homology, whereas the plasmid of one biovar 1A strain partially hybridized to the virulence plasmid.
测定了无致病性小肠结肠炎耶尔森氏菌生物变种1A菌株的质粒含量，并将这些质粒与致病性小肠结肠炎耶尔森氏菌菌株的毒力质粒进行了比较。约38%的选定生物变种1A菌株含有大小不同的质粒，范围从2.7 kb到超过70 kb。一些较大的质粒大小与致病性参考菌株的毒力质粒相似。这些质粒的限制性酶切图谱与致病性参考菌株毒力质粒的限制性酶切图谱不同。在用毒力质粒进行的杂交研究中也观察到了差异。16株生物变种1A菌株中的15株的质粒没有同源性，而一株生物变种1A菌株的质粒与毒力质粒部分杂交。
