Riesgo-Escovar J R, Jenni M, Fritz A, Hafen E
Zoologisches Institut, Universität Zürich, Switzerland.
Genes Dev. 1996 Nov 1;10(21):2759-68. doi: 10.1101/gad.10.21.2759.
We cloned and characterized the Drosophila homolog of mammalian Jun-N-terminal kinases (DJNK). We show that DJNK is encoded by basket (bsk). Like hemipterous (hep), which encodes the Drosophila JNK kinase, bsk is required in the embryo for dorsal closure, a process involving coordinate cell shape changes of ectodermal cells. Dorsal closure can also be blocked by dominant negative Drosophila cdc42, which has been shown to act upstream of JNKK in vertebrates. Therefore it appears that the JNK pathway is conserved and that it is involved in controlling cell morphogenesis in Drosophila. Although DJNK efficiently phosphorylates DJun in vitro, bsk function is not required for the specification of cell fate in the developing eye, a process that requires MAP kinase and DJun function.
我们克隆并鉴定了哺乳动物JNK（Jun氨基末端激酶）的果蝇同源物（DJNK）。我们发现DJNK由basket（bsk）基因编码。与编码果蝇JNK激酶的hemipterous（hep）基因一样，胚胎发育过程中的背侧闭合需要bsk基因，这一过程涉及外胚层细胞协调的细胞形态变化。背侧闭合也可被显性负性果蝇cdc42阻断，在脊椎动物中，cdc42已被证明在JNKK上游发挥作用。因此，JNK信号通路似乎是保守的，并且参与果蝇细胞形态发生的调控。尽管DJNK在体外能有效磷酸化DJun，但在发育中的眼睛中细胞命运的决定并不需要bsk基因的功能，而这一过程需要MAP激酶和DJun的功能。
