Zomas A P, Matutes E, Morilla R, Owusu-Ankomah K, Seon B K, Catovsky D
Academic Department of Haematology and Cytogenetics, Royal Marsden Hospital, London, UK.
Leukemia. 1996 Dec;10(12):1966-70.
We studied the expression of the immunoglobulin-associated membrane protein B29 in 499 cases of chronic B cell diseases using the monoclonal antibody SN8 (CD79b). SN8 was positive in 5% (17/330) of chronic lymphocytic leukemia (CLL) and 100% (15/15) of B prolymphocytic leukemia. The expression of B29 in other B cell disorders was, as a rule, significantly higher than in CLL. Two thirds of non-Hodgkin's lymphomas in leukemic phase were SN8 positive, including lymphoplasmacytic (45%), follicular (83%), mantle cell (92%) and splenic lymphoma with villous lymphocytes (74%) while only 25% of hairy cell leukemias were SN8 positive. Within CLL, 2.3% of typical cases were SN8+ while 16% of cases with atypical morphology and an increased number of prolymphocytes were SN8+. Our results suggest a useful role for SN8 in the immunophenotypic differentiation of B cell disorders as a marker for non-CLL diseases. The analysis of B29 expression may throw light into the structure of the B cell antigen receptor in B cell malignancies while the distinctive reactivity profile of SN8 has direct applications to diagnosis.
我们使用单克隆抗体SN8（CD79b）研究了499例慢性B细胞疾病中免疫球蛋白相关膜蛋白B29的表达情况。SN8在5%（17/330）的慢性淋巴细胞白血病（CLL）和100%（15/15）的B原淋巴细胞白血病中呈阳性。通常，B29在其他B细胞疾病中的表达明显高于CLL。白血病期非霍奇金淋巴瘤中有三分之二SN8呈阳性，包括淋巴浆细胞性淋巴瘤（45%）、滤泡性淋巴瘤（83%）、套细胞淋巴瘤（92%）和伴有绒毛状淋巴细胞的脾淋巴瘤（74%），而毛细胞白血病中只有25%的病例SN8呈阳性。在CLL中，2.3%的典型病例SN8呈阳性，而16%形态不典型且原淋巴细胞数量增加的病例SN8呈阳性。我们的结果表明，SN8作为非CLL疾病的标志物，在B细胞疾病的免疫表型分化中具有重要作用。对B29表达的分析可能有助于深入了解B细胞恶性肿瘤中B细胞抗原受体的结构，而SN8独特的反应谱在诊断中具有直接应用价值。
