Sakurai H, Ohishi T, Fukasawa T
School of Health Sciences, Faculty of Medicine, Kanazawa University, Japan.
FEBS Lett. 1996 Nov 25;398(1):113-9. doi: 10.1016/s0014-5793(96)01219-7.
The GAL11 gene product, which copurifies with RNA polymerase II holoenzyme, is necessary for full expression of many, but not all, genes in yeast. Here we shows that the GAL11 dependence of a gene for expression is determined by the core promoter structure. In the GAL80 gene, a gal11 null mutation caused reduction of TATA-dependent transcription, but exerted no effect on initiator-mediated transcription. GAL11 stimulated TATA-dependent transcription, but did not affect the TATA-independent transcription in HIS4. GAL11 was also required for transcription mediated by a canonical TATA sequence but not by a nonconsensus TATA sequence of HIS3. These results suggest that GAL11 is specifically involved in the transcription machinery formed on the TATA element.
与RNA聚合酶II全酶共同纯化的GAL11基因产物，是酵母中许多（但并非全部）基因充分表达所必需的。我们在此表明，基因表达对GAL11的依赖性由核心启动子结构决定。在GAL80基因中，gal11无效突变导致TATA依赖的转录减少，但对起始子介导的转录没有影响。GAL11刺激TATA依赖的转录，但不影响HIS4中不依赖TATA的转录。由HIS3的典型TATA序列介导的转录需要GAL11，但由其非共有TATA序列介导的转录则不需要。这些结果表明，GAL11特别参与在TATA元件上形成的转录机制。
