del Pino E M, Sanchez G
J Morphol. 1977 Jul;153(1):153-61. doi: 10.1002/jmor.1051530111.
The mature ovary of Gastrotheca riobambae presents large oocytes (3 mm in diameter) of pale yellow color. After ovulation and the beginning of embryonic incubation, the empty postovulatory follicles can be recognized in the ovary for about 30 days. The granulosa of these follicles never fills the follicular lumen and this cavity becomes filled with fluid during the first five days of incubation. Later, at 18 days of incubation, the lumen is mostly empty and contains few cells of the granulosa. Shrinkage results in the disappearance of the follicular cavity by approximately the thirtieth day of incubation. The thecae are thick and become separated by a space. This space is filled progressively with cells, blood capillaries and fluid. After the thirtieth to fortieth day of incubation, these follicles become regressive and disappear. The postovulatory follicles of Gastrotheca may correspond to corpora lutea. The evidence suggests that pouch vascularization and the formation of embryonic chambers of pouch tissue may be under ovarian control. In addition, the process of vitellogenesis is influenced by incubation, as most growth of the ovarian oocyte occurs after birth of the tadpoles. Follicular atresia is common and is similar to that of other frogs.
里奥班巴胃育蛙成熟的卵巢中有直径3毫米的浅黄色大卵母细胞。排卵和胚胎孵化开始后，卵巢中的排卵后空卵泡在约30天内都可被识别。这些卵泡的颗粒层从未充满卵泡腔，在孵化的前五天，这个腔会充满液体。之后，在孵化18天时，腔大部分是空的，仅含有少量颗粒层细胞。萎缩导致卵泡腔在孵化约第30天时消失。卵泡膜很厚，且被一个间隙隔开。这个间隙逐渐充满细胞、毛细血管和液体。孵化第30至40天后，这些卵泡开始退化并消失。里奥班巴胃育蛙的排卵后卵泡可能相当于黄体。有证据表明，育儿袋血管形成和育儿袋组织胚胎腔的形成可能受卵巢控制。此外，卵黄发生过程受孵化影响，因为卵巢卵母细胞的大部分生长发生在蝌蚪出生后。卵泡闭锁很常见，与其他蛙类相似。
