Horneff G, Schou C, Wahn V
Department of Paediatrics, University of Düsseldorf, Germany.
Allergy. 1996 Nov;51(11):842-6. doi: 10.1111/j.1398-9995.1996.tb00032.x.
The study aimed to investigate the possible diagnostic significance of T-cell proliferative responses to purified Der p 1 antigen in allergic children with and without allergic sensitization to house-dust-mite (HDM) allergens. T-cell reactivity to purified Der p 1 antigen was analyzed in 24 children with allergic sensitization to HDM demonstrated by RAST and/or positive skin prick tests. Twelve healthy young adults and 11 children with allergic sensitizations other than HDM served as controls. Of 25 HDM-allergic patients, 16 displayed strong T-cell reactivity to Der p 1 (stimulation indices > 3): nine patients showed no T-cell proliferation despite the presence of specific IgE, and five showed no responses despite positive skin prick test. In two patients, a weak T-cell response to Der p 1 could be demonstrated in the absence of specific IgE and negative skin test result. The two affected subjects did not show evidence of mite allergy. No T-cell responses were observed in adult controls (stimulation indices < 3). We conclude that the assessment of T-cell reactivity to Der p 1 is of little value for the diagnosis of HDM allergy. The importance of T-cell proliferative responses for the study of the pathogenesis of HDM allergy remains unchallenged.
该研究旨在调查对屋尘螨（HDM）过敏原过敏或未过敏的过敏性儿童中，T细胞对纯化的Der p 1抗原的增殖反应的可能诊断意义。对24名经RAST和/或阳性皮肤点刺试验证实对HDM过敏的儿童分析其对纯化的Der p 1抗原的T细胞反应性。12名健康年轻成年人和11名对HDM以外的过敏原过敏的儿童作为对照。在25名HDM过敏患者中，16名对Der p 1表现出强烈的T细胞反应性（刺激指数> 3）：9名患者尽管存在特异性IgE但未显示T细胞增殖，5名患者尽管皮肤点刺试验呈阳性但无反应。在两名患者中，在无特异性IgE和皮肤试验阴性的情况下可证明对Der p 1有微弱的T细胞反应。这两名受影响的受试者未显示螨过敏的证据。在成年对照组中未观察到T细胞反应（刺激指数< 3）。我们得出结论，评估T细胞对Der p 1的反应性对HDM过敏的诊断价值不大。T细胞增殖反应对HDM过敏发病机制研究的重要性仍然没有受到质疑。
