细胞外高迁移率族蛋白1对小鼠红白血病细胞分化至关重要。
Extracellular high-mobility group 1 protein is essential for murine erythroleukaemia cell differentiation.
作者信息
Sparatore B, Passalacqua M, Patrone M, Melloni E, Pontremoli S
机构信息
Institute of Biological Chemistry, University of Genova, Italy.
出版信息
Biochem J. 1996 Nov 15;320 ( Pt 1)(Pt 1):253-6. doi: 10.1042/bj3200253.
Abstract
A high-mobility group 1 (HMG1) protein type isolated from murine erythroleukaemia (MEL) cells promotes acceleration of the differentiation process when added to a MEL cell culture together with the inducer hexamethylene bisacetamide. We now provide direct evidence that the presence of HMG1 protein in the extracellular medium is essential for terminal erythroid differentiation. An extracellular function for HMG1 protein in MEL cell is further supported by a demonstration that this protein is released from MEL cells exposed to the chemical inducer and that the addition of an anti-(HMG1 protein) monoclonal antibody to the cell culture inhibits the differentiation process almost completely. The release of HMG1 protein from MEL cells is modulated by compounds affecting cell calcium homoeostasis, such as a calcium ionophore or verapamil. In fact, in the presence of the ionophore an increased rate of differentiation is accompanied by an enhanced extracellular release of HMG1 protein, whereas in the presence of verapamil both phenomena are significantly decreased.
摘要
从小鼠红白血病（MEL）细胞中分离出的一种高迁移率族1（HMG1）蛋白，当与诱导剂六甲撑双乙酰胺一起添加到MEL细胞培养物中时，可促进分化过程的加速。我们现在提供直接证据表明，细胞外培养基中HMG1蛋白的存在对于终末红系分化至关重要。MEL细胞中HMG1蛋白的细胞外功能进一步得到支持，因为已证明该蛋白从暴露于化学诱导剂的MEL细胞中释放，并且向细胞培养物中添加抗（HMG1蛋白）单克隆抗体几乎完全抑制分化过程。影响细胞钙稳态的化合物，如钙离子载体或维拉帕米，可调节HMG1蛋白从MEL细胞中的释放。事实上，在存在离子载体的情况下，分化速率增加伴随着HMG1蛋白细胞外释放增强，而在存在维拉帕米的情况下，这两种现象均显著降低。
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