Brons N H, Blaich A, Wiesmüller K H, Schneider F, Jung G, Muller C P
Laboratorie National de Santé, Luxembourg, Germany.
Scand J Immunol. 1996 Nov;44(5):478-84. doi: 10.1046/j.1365-3083.1996.d01-336.x.
The induction of antibodies against peptides requires the presence of a T helper cell epitope. In the absence of an added T-cell epitope only 10% of the mice, or less depending on the strain, gave an antibody response to a series of peptides of the measles virus (MV) fusion (F) protein. After coimmunization with a non-covalently coupled T-cell epitope more than 60% of the peptides became immunogenic. Considerable differences became apparent when BALB/c mice were immunized with peptides in the presence of different T-cell epitopes. An immunodominant T-cell epitope of the MV-F protein was more efficient than a subdominant or a cryptic T-cell epitope in providing help to a non-linked B-cell epitope. There is both a ranking order of the amount of help which B-cell epitopes require and a ranking order for the help T-cell epitopes are able to provide. The capability of a T-cell epitope to provide help to a B-cell epitope correlated with its own immunogenicity, i.e. the intensity of the antibody response to the peptide representing the T-cell epitope. The data suggest that for each MHC class II allele there is an optimal T-cell epitope which can provide help to a maximal number of B-cell epitopes and that such a peptide can be identified by its ability to induce antibodies against itself. By using this strategy, the authors were able to induce antibodies which cross-reacted with the MV.
针对肽段诱导抗体需要存在T辅助细胞表位。在没有添加T细胞表位的情况下，只有10%的小鼠（或更少，取决于品系）对一系列麻疹病毒（MV）融合（F）蛋白肽段产生抗体反应。与非共价偶联的T细胞表位共同免疫后，超过60%的肽段变得具有免疫原性。当用不同T细胞表位存在下的肽段免疫BALB/c小鼠时，明显出现了相当大的差异。MV-F蛋白的免疫显性T细胞表位在为非连锁B细胞表位提供帮助方面比次显性或隐蔽性T细胞表位更有效。B细胞表位所需的帮助量存在一个排序，T细胞表位能够提供的帮助也存在一个排序。T细胞表位为B细胞表位提供帮助的能力与其自身的免疫原性相关，即对代表T细胞表位的肽段的抗体反应强度。数据表明，对于每个MHC II类等位基因，都有一个最佳T细胞表位，它可以为最大数量的B细胞表位提供帮助，并且这样的肽段可以通过其诱导针对自身的抗体的能力来鉴定。通过使用这种策略，作者能够诱导出与MV发生交叉反应的抗体。
