Härkönen M, Tarkkanen A
Acta Ophthalmol (Copenh). 1976 Aug;54(4):445-55. doi: 10.1111/j.1755-3768.1976.tb01276.x.
Steady-state concentrations of the key intermediates from the glycolytic, pentose phosphate, and sorbitol pathways as well as the pyridine nucleotides were measured from the lens after 0.25% phospholine iodide had been instilled into rabbits' eyes twice a day for 18 weeks. In the lenses of those rabbits which had received treatment in both eyes fructose-1,6-diphosphate and pyruvate levels were increased, whereas 6-phosphogluconate, sorbitol and alpha-glycer0phosphate concentrations were decreased. alpha-Ketoglutarate and concentrations and ratios of NAD+ and NADH did not show any changes. In contrast, NADPH and total NADP concentrations as well as the NADPH/NADP+ ratio were decreased, and therefore total NAD/total NADP ratio increased after treatment. It appears that instillation of long-acting 0.25% phospholine iodide into rabbits' eyes results in increased glycolytic activity in the lens in response to the increased energy demand, wheras the activities of other metabolic pathways are suppressed.
在每天给兔子双眼滴注0.25%碘化磷酰硫胆碱，持续18周后，测定了晶状体中糖酵解途径、磷酸戊糖途径、山梨醇途径的关键中间体以及吡啶核苷酸的稳态浓度。在双眼均接受治疗的兔子晶状体中，1,6 -二磷酸果糖和丙酮酸水平升高，而6 -磷酸葡萄糖酸、山梨醇和α -甘油磷酸浓度降低。α -酮戊二酸以及NAD⁺和NADH的浓度及比率没有变化。相反，NADPH和总NADP浓度以及NADPH/NADP⁺比率降低，因此治疗后总NAD/总NADP比率升高。似乎向兔子眼中滴注长效0.25%碘化磷酰硫胆碱会导致晶状体中糖酵解活性增加，以应对能量需求的增加，而其他代谢途径的活性受到抑制。
