Morrone G, Bond H M, Cuomo C, Agosti V, Petrella A, Pagnano A M, Della Corte A, Marasco O, Venuta S
Department of Experimental and Clinical Medicine, Faculty of Medicine, Catanzaro, Italy.
Biochem J. 1995 Jun 15;308 ( Pt 3)(Pt 3):909-14. doi: 10.1042/bj3080909.
The common gamma-chain (gamma c) is a shared component of cell-surface receptors for the interleukins- 2, -4 and -7, and possibly others. We studied its expression in cells and cell lines of myeloid origin and found ubiquitous presence of gamma c mRNA in all cells examined. Differential regulation of gamma c expression was observed in myeloid cell lines induced to differentiate in vitro. In K-562 erythromyeloid cells, a sharp rise in the levels of gamma c mRNA and protein accompanied megakaryocytic, but not erythroid, differentiation. Surface binding of interleukin-2, as well as the transcripts for cognate receptor chains, were scarcely detectable in K-562 cells, whereas a significant increase in the binding of granulocyte-macrophage colony-stimulating factor specifically occurred during their megakaryocytic maturation. Our data indicate that expression of gamma c is a common feature of human myeloid cells, and suggest that its expression may be a requirement for human myelopoiesis.
共同γ链（γc）是白细胞介素-2、-4和-7以及可能其他细胞表面受体的共享成分。我们研究了其在髓系来源的细胞和细胞系中的表达，发现在所有检测的细胞中均普遍存在γc mRNA。在体外诱导分化的髓系细胞系中观察到γc表达的差异调节。在K-562红髓系细胞中，γc mRNA和蛋白质水平的急剧上升伴随着巨核细胞而非红细胞的分化。在K-562细胞中几乎检测不到白细胞介素-2的表面结合以及同源受体链的转录本，而在其巨核细胞成熟过程中，粒细胞-巨噬细胞集落刺激因子的结合显著增加。我们的数据表明γc的表达是人类髓系细胞的共同特征，并表明其表达可能是人类髓系造血的必要条件。
