Perkins L A, Johnson M R, Melnick M B, Perrimon N
Pediatric Surgical Research Laboratories, Massachusetts General Hospital, Harvard Medical School, Boston 02114, USA.
Dev Biol. 1996 Nov 25;180(1):63-81. doi: 10.1006/dbio.1996.0285.
Corkscrew (csw) encodes a nonreceptor protein tyrosine phosphatase (PTPase) that has been implicated in signaling from the Torso receptor tyrosine kinase (RTK). csw mutations, unlike tor mutations, are associated with zygotic lethality, indicating that Csw plays additional roles during development. We have conducted a detailed phenotypic analysis of csw mutations to identify these additional functions of Csw. Our results indicate that Csw operates positively downstream of other Drosophila RTKs such as the Drosophila epidermal growth factor receptor (DER), the fibroblast growth factor receptor (Breathless), and likely other RTKs. This model is substantiated by specific dosage interactions between csw and DER. It is proposed that Csw is part of the evolutionarily conserved "signaling cassette" that operates downstream of all RTKs. In support of this hypothesis, we demonstrate that SHP-2, a vertebrate PTPase similar to Csw and previously implicated in RTK signaling, encodes the functional vertebrate homologue of Csw.
螺旋（csw）编码一种非受体蛋白酪氨酸磷酸酶（PTPase），该酶与躯干受体酪氨酸激酶（RTK）的信号传导有关。与tor突变不同，csw突变与合子致死有关，这表明Csw在发育过程中发挥额外作用。我们对csw突变进行了详细的表型分析，以确定Csw的这些额外功能。我们的结果表明，Csw在其他果蝇RTK（如有果蝇表皮生长因子受体（DER）、成纤维细胞生长因子受体（呼吸急促）以及可能的其他RTK）下游发挥正向作用。csw与DER之间的特定剂量相互作用证实了这一模型。有人提出，Csw是在所有RTK下游起作用的进化保守“信号盒”的一部分。为支持这一假设，我们证明，SHP-2，一种与Csw相似且先前与RTK信号传导有关的脊椎动物PTPase，编码Csw的功能性脊椎动物同源物。
