酿酒酵母II类转录基础抑制剂NC2的特性分析。
Characterization of the basal inhibitor of class II transcription NC2 from Saccharomyces cerevisiae.
作者信息
Goppelt A, Meisterernst M
机构信息
Laboratorium für Molekulare Biologie-Genzentrum der Ludwig-Maximilians-Universität München, Germany.
出版信息
Nucleic Acids Res. 1996 Nov 15;24(22):4450-5. doi: 10.1093/nar/24.22.4450.
Abstract
Human NC2 utilizes a unique mechanism of repression of transcription by associating with TBP and inhibition of preinitiation complex formation. Here we have cloned two genes from Saccharomyces cerevisiae and functionally characterized them as yeast NC2. We show that yeast NC2 binds to TBP as a heterodimer and represses RNA polymerase II transcription during assembly of the preinitiation complex. Yeast NC2 is highly homologous to its human counterpart within histone fold domains. C-Terminal regions previously discussed to be important for repression in man are in part not conserved. The human alpha but not the beta subunit efficiently heterodimerizes and represses transcription in combination with the corresponding yeast subunit. Yeast and human NC2 inhibit transcription in the presence of yeast and human TBP. However, repression is optimal within one species. The N-terminus of human TBP supports repression of transcription by human but not by yeast NC2.
摘要
人类NC2通过与TBP结合并抑制起始前复合物的形成，利用一种独特的转录抑制机制。在这里，我们从酿酒酵母中克隆了两个基因，并对其进行功能鉴定，确定它们为酵母NC2。我们发现酵母NC2作为异二聚体与TBP结合，并在起始前复合物组装过程中抑制RNA聚合酶II转录。酵母NC2在组蛋白折叠结构域内与其人类对应物高度同源。先前讨论的对人类抑制作用很重要的C末端区域部分并不保守。人类α亚基而非β亚基能与相应的酵母亚基有效异二聚化并抑制转录。酵母和人类NC2在存在酵母和人类TBP的情况下均抑制转录。然而，抑制作用在同一物种内最为理想。人类TBP的N末端支持人类而非酵母NC2对转录的抑制作用。
相似文献
1
Characterization of the basal inhibitor of class II transcription NC2 from Saccharomyces cerevisiae.酿酒酵母II类转录基础抑制剂NC2的特性分析。
Nucleic Acids Res. 1996 Nov 15;24(22):4450-5. doi: 10.1093/nar/24.22.4450.
2
A single point mutation in TFIIA suppresses NC2 requirement in vivo.TFIIA中的单个点突变在体内抑制了对NC2的需求。
EMBO J. 2000 Feb 15;19(4):672-82. doi: 10.1093/emboj/19.4.672.
3
4
Yeast NC2 associates with the RNA polymerase II preinitiation complex and selectively affects transcription in vivo.酵母NC2与RNA聚合酶II起始前复合物结合，并在体内选择性地影响转录。
Mol Cell Biol. 2001 Apr;21(8):2736-42. doi: 10.1128/MCB.21.8.2736-2742.2001.
5
Direct stimulation of transcription by negative cofactor 2 (NC2) through TATA-binding protein (TBP).负辅因子2（NC2）通过TATA结合蛋白（TBP）直接刺激转录。
Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12727-32. doi: 10.1073/pnas.202236699. Epub 2002 Sep 17.
6
The NC2 repressor is dispensable in yeast mutated for the Sin4p component of the holoenzyme and plays roles similar to Mot1p in vivo.在全酶的Sin4p组分发生突变的酵母中，NC2阻遏物是可有可无的，并且在体内发挥与Mot1p类似的作用。
Mol Microbiol. 2000 Apr;36(1):163-73. doi: 10.1046/j.1365-2958.2000.01839.x.
7
Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast.NC2 阻遏物的缺陷会影响酵母中经典和非编码 RNA 聚合酶 II 的转录起始。
BMC Genomics. 2016 Mar 3;17:183. doi: 10.1186/s12864-016-2536-2.
8
Nonradioactive, ultrasensitive site-specific protein-protein photocrosslinking: interactions of alpha-helix 2 of TATA-binding protein with general transcription factor TFIIA and transcriptional repressor NC2.非放射性、超灵敏位点特异性蛋白质-蛋白质光交联：TATA结合蛋白的α螺旋2与通用转录因子TFIIA及转录抑制因子NC2的相互作用
Nucleic Acids Res. 2008 Nov;36(19):6143-54. doi: 10.1093/nar/gkn612. Epub 2008 Sep 29.
9
Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex.识别TBP-DNA转录复合物的负辅因子2的晶体结构。
Cell. 2001 Jul 13;106(1):71-81. doi: 10.1016/s0092-8674(01)00417-2.
10
The NC2 alpha and beta subunits play different roles in vivo.NC2的α亚基和β亚基在体内发挥不同的作用。
Genes Dev. 2002 Dec 15;16(24):3265-76. doi: 10.1101/gad.234002.
引用本文的文献
1
Transcription factor VIB-1 activates catalase-3 expression by promoting PIC assembly in Neurospora crassa.转录因子VIB-1通过促进粗糙脉孢菌中的预起始复合物组装来激活过氧化氢酶-3的表达。
Nucleic Acids Res. 2025 Feb 27;53(5). doi: 10.1093/nar/gkaf174.
2
NC2 complex is a key factor for the activation of catalase-3 transcription by regulating H2A.Z deposition.NC2 复合物是通过调节 H2A.Z 沉积来激活过氧化氢酶-3 转录的关键因素。
Nucleic Acids Res. 2020 Sep 4;48(15):8332-8348. doi: 10.1093/nar/gkaa552.
3
The negative cofactor 2 complex is a key regulator of drug resistance in Aspergillus fumigatus.负辅助因子 2 复合物是烟曲霉中药物耐药性的关键调节剂。
Nat Commun. 2020 Jan 22;11(1):427. doi: 10.1038/s41467-019-14191-1.
4
A meta-analysis reveals complex regulatory properties at Taf14-repressed genes.一项荟萃分析揭示了Taf14抑制基因的复杂调控特性。
BMC Genomics. 2017 Feb 16;18(1):175. doi: 10.1186/s12864-017-3544-6.
5
Defects in the NC2 repressor affect both canonical and non-coding RNA polymerase II transcription initiation in yeast.NC2 阻遏物的缺陷会影响酵母中经典和非编码 RNA 聚合酶 II 的转录起始。
BMC Genomics. 2016 Mar 3;17:183. doi: 10.1186/s12864-016-2536-2.
6
Insights into mRNP biogenesis provided by new genetic interactions among export and transcription factors.新的外排和转录因子遗传相互作用提供了对 mRNP 生物发生的深入了解。
BMC Genet. 2012 Sep 10;13:80. doi: 10.1186/1471-2156-13-80.
7
Tight cooperation between Mot1p and NC2β in regulating genome-wide transcription, repression of transcription following heat shock induction and genetic interaction with SAGA.Mot1p 和 NC2β 在调控全基因组转录、热激诱导后转录抑制以及与 SAGA 的遗传相互作用方面的紧密合作。
Nucleic Acids Res. 2012 Feb;40(3):996-1008. doi: 10.1093/nar/gkr784. Epub 2011 Oct 5.
8
Ncb2 is involved in activated transcription of CDR1 in azole-resistant clinical isolates of Candida albicans.Ncb2参与白色念珠菌唑类耐药临床分离株中CDR1的激活转录。
Eukaryot Cell. 2011 Oct;10(10):1357-66. doi: 10.1128/EC.05041-11. Epub 2011 Aug 19.
9
Dr1 (NC2) is present at tRNA genes and represses their transcription in human cells.DR1（NC2）位于 tRNA 基因附近，并在人体细胞中抑制其转录。
Nucleic Acids Res. 2010 Mar;38(4):1228-39. doi: 10.1093/nar/gkp1102. Epub 2009 Dec 3.
10
The yeast RNA polymerase II-associated factor Iwr1p is involved in the basal and regulated transcription of specific genes.酵母RNA聚合酶II相关因子Iwr1p参与特定基因的基础转录和调控转录。
J Biol Chem. 2009 Oct 16;284(42):28958-67. doi: 10.1074/jbc.M109.012153. Epub 2009 Aug 13.
本文引用的文献
1
Determination of functional domains in the C subunit of the CCAAT-binding factor (CBF) necessary for formation of a CBF-DNA complex: CBF-B interacts simultaneously with both the CBF-A and CBF-C subunits to form a heterotrimeric CBF molecule.确定CCAAT结合因子（CBF）C亚基中形成CBF-DNA复合物所必需的功能结构域：CBF-B同时与CBF-A和CBF-C亚基相互作用，形成异源三聚体CBF分子。
Mol Cell Biol. 1996 Aug;16(8):4003-13. doi: 10.1128/MCB.16.8.4003.
2
3
Crystal structure of the yeast TFIIA/TBP/DNA complex.酵母TFIIA/TBP/DNA复合物的晶体结构。
Science. 1996 May 10;272(5263):830-6. doi: 10.1126/science.272.5263.830.
4
Crystal structure of a yeast TFIIA/TBP/DNA complex.酵母TFIIA/TBP/DNA复合物的晶体结构
Nature. 1996 May 9;381(6578):127-51. doi: 10.1038/381127a0.
5
Requirement of a corepressor for Dr1-mediated repression of transcription.转录抑制因子Dr1介导转录抑制所需的共抑制因子。
Genes Dev. 1996 Apr 15;10(8):1033-48. doi: 10.1101/gad.10.8.1033.
6
A histone octamer-like structure within TFIID.转录因子IID内的一种组蛋白八聚体样结构。
Nature. 1996 Mar 28;380(6572):356-9. doi: 10.1038/380356a0.
7
Structural similarity between TAFs and the heterotetrameric core of the histone octamer.TAFs与组蛋白八聚体异源四聚体核心之间的结构相似性。
Nature. 1996 Mar 28;380(6572):316-22. doi: 10.1038/380316a0.
8
Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex.CCAAT结合因子CBF的A亚基中的三类突变描绘了参与CBF-DNA复合物三步组装的功能结构域。
Mol Cell Biol. 1996 Jan;16(1):328-37. doi: 10.1128/MCB.16.1.328.
9
Factors (TAFs) required for activated transcription interact with TATA box-binding protein conserved core domain.激活转录所需的因子（TAFs）与TATA盒结合蛋白保守核心结构域相互作用。
Genes Dev. 1993 Feb;7(2):180-7. doi: 10.1101/gad.7.2.180.
10
Topography of the histone octamer surface: repeating structural motifs utilized in the docking of nucleosomal DNA.组蛋白八聚体表面的拓扑结构：核小体DNA对接中使用的重复结构基序。
Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10489-93. doi: 10.1073/pnas.90.22.10489.
Zotero 插件