Raaphorst G P, Yang H, Wilkins D E, Ng C E
Ottawa Regional Cancer Centre, Ontario, Canada.
Int J Hyperthermia. 1996 Nov-Dec;12(6):801-12. doi: 10.3109/02656739609027686.
In this study, the effects of mild protracted hyperthermia, combined with prolonged exposure to cisplatin and low dose-rate irradiation (LDRI), were examined in two human cell lines. The cell lines are human glioma parental and cisplatin-resistant variant cells. The results show that mild hyperthermia at 40 degrees C was able to sensitize both the parental and the variant cisplatin-resistant cells to cisplatin treatments (1 microgram/ml for up to 20 h) when the two treatments were given concurrently. When mild hyperthermia and cisplatin were given with LDRI concurrently, additional enhanced cell killing was observed in both the parental and the cisplatin-resistant variant cells. Further analysis of the results showed that when the effects of the trimodality treatment were normalized to the effects of the combined treatment of mild hyperthermia with cisplatin, the residual cell killing was still greater than that observed for radiation alone, indicating a synergistic interaction. This synergistic interaction was greater for the parental line compared to the cisplatin-resistant line. Thus, these data show that the concurrent application of mild hyperthermia, low concentration, long duration, cisplatin and low-dose rate irradiation may be an effective form of treatment in both normally responding and cisplatin-resistant variant human tumour cell lines.
在本研究中，在两种人类细胞系中检测了轻度持续性热疗联合长时间暴露于顺铂和低剂量率照射（LDRI）的效果。这两种细胞系分别是人类胶质瘤亲本细胞和顺铂耐药变异细胞。结果表明，当两种处理同时进行时，40℃的轻度热疗能够使亲本细胞和顺铂耐药变异细胞对顺铂处理（1微克/毫升，持续20小时）敏感。当轻度热疗和顺铂与LDRI同时进行时，在亲本细胞和顺铂耐药变异细胞中均观察到额外的增强细胞杀伤作用。对结果的进一步分析表明，当将三联疗法的效果归一化为轻度热疗与顺铂联合治疗的效果时，残留的细胞杀伤作用仍大于单独放疗时观察到的效果，表明存在协同相互作用。与顺铂耐药细胞系相比，亲本细胞系的这种协同相互作用更大。因此，这些数据表明，同时应用轻度热疗、低浓度、长时间的顺铂和低剂量率照射可能是一种对正常反应和顺铂耐药变异人类肿瘤细胞系均有效的治疗形式。
