Isolation and characterization of the Bacillus subtilis tryptophanyl-tRNA synthetase gene (trpS) conferring 5-fluorotryptophan resistance and temperature sensitivity.
作者信息
Chow K C, Wong J T
机构信息
Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, Hong Kong.
出版信息
Biochim Biophys Acta. 1996 Nov 11;1309(1-2):42-6. doi: 10.1016/s0167-4781(96)00136-4.
PMID:8950174
Abstract
The mutant trpS gene of Bacillus subtilis encoding a thermosensitive tryptophanyl-tRNA synthetase that also confers resistance to growth inhibition by 5-fluorotryptophan at permissive temperature has been isolated and characterized. A point mutation at codon 82 of the gene from a wild-type TCA codon for Ser to a TTA codon for Leu has been identified.
摘要
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